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| 马铃薯多酚氧化酶的特性研究 | |
| 引用本文： | 李敏,刘磊,郭玉蓉,陈德蓉,李永才.马铃薯多酚氧化酶的特性研究[J].甘肃农业大学学报,2005,40(2):189-192. |
| 作者姓名： | 李敏 刘磊 郭玉蓉 陈德蓉 李永才 |
| 作者单位： | 1. 甘肃农业大学食品科学与工程学院,甘肃,兰州,730070 2. 甘肃农业大学动物医学院,甘肃,兰州,730070 3. 甘肃农业大学生命科学院,甘肃,兰州,730070 |
| 基金项目： | 甘肃省自然基金资助(编号:ZS011-A25-032-N)。 |
| 摘 要： | 多酚氧化(PPO)酶是导致切分马铃薯变色的主要原因。以大西洋马铃薯为材料,采用分光光度法研究其PPO的特性。结果表明:该酶仅作用于邻苯酚,与对苯酚、间苯酚和一元酚无作用。以邻苯二酚为底物,该酶的最适pH值为5.5,最适温度为5℃。除氯化钠和EDTA外,供试的其它5种浓度为0.01mol/L的抑制剂均可不同程度地对该酶产生抑制。 |
| 关 键 词： | 马铃薯 多酚氧化酶 褐变 |
| 文章编号： | 1003-4315(2005)02-0189-04 |
Properties of polyphenol oxidase extracted from potato |
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| LI Min,LIU Lei,GUO Yu-rong,CHEN De-rong,LI Yong-cai.Properties of polyphenol oxidase extracted from potato[J].Journal of Gansu Agricultural University,2005,40(2):189-192. | |
| Authors: | LI Min LIU Lei GUO Yu-rong CHEN De-rong LI Yong-cai |
| Institution: | LI Min1,LIU Lei2,GUO Yu-rong1,CHEN De-rong3,LI Yong-cai1 |
| Abstract: | The discoloration of fresh-cut potato was mainly due to its PPO. The processed potato cultivar Atlantic was used to study its PPO property by spectrocolorimetry. The results showed that the substrate of PPO was o-diphenol, but not hydroquinone, m-diphenol, and mono-phenol. The optimum pH value was 5.5 and the optimum temperature was 5 ℃ for PPO with o-diphenol as a substrate. The chosen inhibitors at 0.01mol/L restrained PPO except NaCl2 and EDTA. |
| Keywords: | potato polyphenol oxidase discoloration |
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