三株猪2型圆环病毒甘肃分离株的全基因组序列分析

根据GenBank中发表的猪2型圆环病毒(PCV2)全基因组序列,设计合成了2对引物,采用PCR方法对来自甘肃不同猪场的3份病料中的PCV2进行基因的扩增、克隆和测序,得到全基因组长度为1 768 bp的PCV2Ww株(登录号DQ322701)以及全基因组长度为1 767 bp的PCV2 LZ株和TS株(登录号DQ363860和DQ355153).应用DNAstar软件序列分析可得,3个分离株全基因组与国内外参考毒株核苷酸序列同源性在94.8%~99.4%,3个分离株之间的核苷酸序列同源性为96.4%~99.4%;PCV2分离株与PCV1分离株核苷酸序列同源性为69.0%~70.0%;ORF1和ORF2所编码的氨基酸序列与国内外PCV2毒株比较,同源性也很高,分别为99.0%~99.4%和92.7%~98.7%.进化树分析表明,PCV2分离毒株在进化上存在地域上的相关性.

Sequence analysis of three porcine circovirus type 2 strains from Gansu province

According to the sequence of porcine circovirus type 2(PCV2) published in Genbank,the primers were designed to amplify the complete genome of PCV2 from the suspected PMWS three samples by PCR.The complete genome of PCV2 Ww strain was 1 768 bp,and LZ and TS strain were both 1 767 bp.The sequences were loaded to the GenBank and acquired three logging number: DQ322701,DQ363860 and DQ355153.The three sequences were compared with other published genomes of PCVs by DNAstar.The results showed that the sequences of the three isolates were closely related to those of other PCV2 isolates in Genbank,homology were 94.8 % and 99.4 %.The homology of nucleotide sequences of the three isolates were between 96.4 % and 99.4 %.However there were only 69.0 %～70 % nucleotide identities with the PCV1 isolates.The amino acid sequences of ORF1 and ORF2 of the isolates showed high identities,from 99% to 99.4 % and 92.7 % to 98.7 %.Phylogenetic tree analysis showed that there was a regional correlation among PCV2 isolates.