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甘肃省河西地区菜豆病毒病分子检测
引用本文:文朝慧,王军平,张丽萍,周婷,火克琴,黄超杰,侯建雄.甘肃省河西地区菜豆病毒病分子检测[J].甘肃农业大学学报,2012,47(6).
作者姓名:文朝慧  王军平  张丽萍  周婷  火克琴  黄超杰  侯建雄
作者单位:甘肃出入境检验检疫局,甘肃兰州,730010
基金项目:甘肃出入境检验检疫局科技计划项目
摘    要:以采集自甘肃省河西地区表现病毒病症状的菜豆病株叶组织总RNA为模板,进行RT-PCR扩增,鉴定出苜蓿花叶病毒、小西葫芦黄花叶病毒、菜叶普通花叶病毒、黑眼豇豆花叶病毒4种病毒.对预期大小的扩增产物进行直接测序.结果表明:该地区菜豆病毒病的发生以苜蓿花叶病毒、黑眼豇豆花叶病毒和小西葫芦黄花叶病毒复合侵染为主.

关 键 词:菜豆  病毒病  分子诊断  复合侵染

Molecular diagnosis and detection of mix-infection of viruses on Phaseolus vulgaris in Gansu Province
WEN Zhao-hui , WANG Jun-ping , ZHANG Li-ping , ZHOU Ting , HUO Ke-qin , HUANG Chao-jie , HOU Jian-xiong.Molecular diagnosis and detection of mix-infection of viruses on Phaseolus vulgaris in Gansu Province[J].Journal of Gansu Agricultural University,2012,47(6).
Authors:WEN Zhao-hui  WANG Jun-ping  ZHANG Li-ping  ZHOU Ting  HUO Ke-qin  HUANG Chao-jie  HOU Jian-xiong
Abstract:There were three viruses which were alfalfa mosaic virus,blackeye cowpea mosaic virus and zucchini yellow mosaic virus detected in the infected bean samples collected from Hexi Region in Gansu Province.RT-PCR was performed with the total RNA as templates extracted from diseased bean(Phaseolus vulgaris) leafages.The amplified DNA fragments with expected sizes were sequenced.The nucleotide sequences of DNA fragments amplified from the samples with virus-specific primers had highly homology to the CP gene of their corresponding isolates around the world.Results indicated that two or three viruses cause mix-infection on bean.
Keywords:Phaseolus vulgaris  virus  molecular diagnosis  mix-infection
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