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猪圆环病毒Ⅱ型特异抗体检测间接ELISA法的建立
引用本文:魏凡华,曹瑞兵,张玉颖,吴润,吴延功,张晓勇,陈溥言.猪圆环病毒Ⅱ型特异抗体检测间接ELISA法的建立[J].甘肃农业大学学报,2006,41(6):10-13.
作者姓名:魏凡华  曹瑞兵  张玉颖  吴润  吴延功  张晓勇  陈溥言
作者单位:1. 甘肃农业大学动物医学院,甘肃,兰州,730070;南京农业大学农业部疫病诊断与免疫重点开放实验室,江苏,南京,210095;宁夏大学,宁夏,银川,750000
2. 南京农业大学农业部疫病诊断与免疫重点开放实验室,江苏,南京,210095
3. 甘肃农业大学动物医学院,甘肃,兰州,730070
4. 农业部动物检疫所,山东,青岛,266000
摘    要:利用巴斯德毕赤酵母表达的猪圆环病毒2型株Cap蛋白为包被抗原,成功地建立了一种检测血清中PCV-2特异抗体的间接ELISA方法.确定了血清样品阴、阳性判定标准.临床检测结果表明,自建ELISA试剂盒具有较高的敏感性和特异性.

关 键 词:猪圆环病毒Ⅱ型  重组Cap蛋白  间接ELISA
文章编号:1003-4315(2006)06-0010-04
修稿时间:2006年3月3日

Development of indirect-ELISA method for detecting specific antibody of type Ⅱ porcine circovirus
WEI Fan-hua,CAO Rui-bing,ZHANG Yu-ying,WU Run,WU Yan-gong,ZHANG Xiao-yong,CHEN Pu-yan.Development of indirect-ELISA method for detecting specific antibody of type Ⅱ porcine circovirus[J].Journal of Gansu Agricultural University,2006,41(6):10-13.
Authors:WEI Fan-hua  CAO Rui-bing  ZHANG Yu-ying  WU Run  WU Yan-gong  ZHANG Xiao-yong  CHEN Pu-yan
Abstract:The recombinant Cap protein of type Ⅱ porcine circovirus expressed in Pichia pastoris was used as antigen for developing an directed-ELISA assay to detect PCV-2 specific antibody.Conditions were optimized for using these preparations for an PCV-2 specific ELISA.Following the determination of conditions of ELISA,the standard value of ELISA was defied.Serum samples from porcine were detected for the antibody to PCV-2 by using the developed ELISA.It was found that the specificity and sensitivity of the developed ELISA were quite well,and can differentiate the standard negative serum and positive serum notably.
Keywords:type Ⅱ porcine circovirus  recombinant Cap protein  indirect-ELISA
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