LED不同光质对葡萄砧木试管苗生理生化特性的影响

以葡萄砧木‘贝达’和变叶葡萄试管苗为试验材料,对其在LED白光、红光、黄光、蓝光、绿光和蓝红光6种光质,16h·d-1、12h·d-1、8h·d-1、24h·d-14个光周期下叶片色素含量、气孔差异性、SOD活性和光合速率进行了研究.结果表明:2种试验材料在不同的LED光质和光周期下均表现出气孔频度越大,气孔长×宽越小,其中,变叶葡萄气孔频度在蓝光光周期为16h·d-1条件下最高,气孔长×宽最小,而‘贝达’试管苗气孔频度在蓝红光光周期为24h·d-1条件下最高,气孔长×宽最小.红光、蓝光和蓝红光有利于叶绿素的合成,但8h·d-1光周期叶绿素合成能力差.2种试管苗SOD活性均在蓝光下最高,红光下最低,其中,‘贝达’试管苗在8h·d-1光周期下最高,24h光周期条件下最低,变叶葡萄试管苗则在16h光周期条件下最高,12h光周期下最低.2种砧木试管苗均在LED蓝红光下光合速率最大,而在LED黄光和绿光下均为负值.

Effects of different LED light on physiological and biochemical characters of grape rootstock plantlets

Two grape rootstock plantlets(cvs.Beta and VitispiasezkiiMaxim)were used as material to study the effect of changes of light qualities(white,red,yellow,blue,green and blue+red)and photoperiods(16h·d-1,12h·d-1,8h·d-1,24h·d-1)on leaf pigment content,stomatal difference,SOD activity and photosynthetic rate.The results showed that a bigger stomatal frequencies had a smaller stomatal length×width under different light qualities and photoperiods.V.piasezkii had the maximum stomatal frequency and the minimum stomatal length×width under blue irradiation and 16h·d-1.'Beta'had the maximum stomatal frequency and the minimum stomatal length×width under blue+red irradiation and 24h·d-1.Red,blue and blue+red light showed benefit to chlorophyll synthesis,and 8h·d-1 was inferior to synthesize chlorophyll.SOD activity of both plantlets were the highest under blue light and lowest under red light,and the highest of'Beta'and V.piasezkii was under 8h·d-1,16h·d-1 respectively,the lowest under 24h and 12h irradiation respectively.The photosynthetic rate of two plantlets were the maximum under the blue+red LED light,however that were negative under the yellow and green LED light.