| 葡萄多倍体诱导及其特征特性研究 |
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| 引用本文： | 杨晓明,王翠玲.葡萄多倍体诱导及其特征特性研究[J].甘肃农业大学学报,2005,40(6):741-744. |
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| 作者姓名： | 杨晓明 王翠玲 |
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| 作者单位： | 1. 甘肃农业科学院粮食作物研究所,甘肃,兰州,730070
2. 甘肃农业大学生命科学技术学院,甘肃,兰州,730070 |
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| 摘 要： | 通过葡萄的离体培养,对多倍体葡萄诱导及其特征特性进行了研究,结果表明,秋水仙素加入培养基中诱导葡萄多倍体,以20 mg／L秋水仙素加入附加有0．01 mg／L 6-BA的MS培养基中包埋腋芽诱导染色体加倍效果较好。不同品种对秋水仙素的敏感反应不一,以红提为亲本诱导获得染色体加倍率分别为66．1％、64．6％的诱变株系 H0120-1和H0120-3。诱变株与原二倍体红提比较,具有多倍体的部分特征特性;诱变株与原二倍体红提气孔平均大小、气孔指数无明显差异,但诱变株存在20．8％的特大气孔。
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| 关 键 词： | 葡萄 组织培养 多倍体 秋水仙素 |
| 文章编号： | 1003-4315（2005）06-0741-04 |
| 收稿时间： | 2005-04-12 |
| 修稿时间： | 2005-04-12 |
Studies on induction and characteristics of grape polyploid |
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| YANG Xiao-ming,WANG Cui-ling.Studies on induction and characteristics of grape polyploid[J].Journal of Gansu Agricultural University,2005,40(6):741-744. |
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| Authors: | YANG Xiao-ming WANG Cui-ling |
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| Institution: | 1. Crop Institute, Gansu Academy of Agricultural Sciences,Gansu,Lanzhou 730070;2. College of Life Science and Technology, Gansu Agricultural University,Gansu,Lanzhou 730070, China |
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| Abstract: | Polyploid induction and its characteristics of grape were studied. The results showed that the optimum way to produce tetraploid plant was inducing auxiliary buds on MS supplement with colchi cine 20 mg/L and BA 0. 01 mg/L for 12 days. Effects of induction depended on different varieties. The two induced plants,H120-1 and H120-3,with chromosome doubling rate 66. 1 % and 64. 6 Irrespectively, h-ad been obtained by inducing diploid cultivar Red Globe on MS with colchicines. Compared with the donor plants,the lines had some typical polyploid characteristics. Studies on stoma showed that the differences of average stoma size and stoma indexes between the donors and the induced plants were nonsignificant, but 20. 8 % stomas of induced plants were abnormally big. |
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| Keywords: | grape tissue culture polyploid colchicines |
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