orf224基因与芸薹属种间杂种雄性不育性的相关性

orf224是在甘蓝型油菜(Brassica napus)波里马(Polima)胞质中发现的一个与细胞质雄性不育(Cytoplasmicmale sterility,CMS)相关的线粒体基因.大白菜(B.campestrisssp.pekinensis)CMS系CMS3411-7是由Polima不育系和大白菜自交系3411-7杂交后,以3411-7为轮回亲本经多代回交选育得到的.以CMS3411-7为母本分别与小白菜(B.campestrisssp.chinensis)自交系S71、大白菜自交系K1和菜心(B.campestrisssp.chinensisvar.utilis)自交系B1杂交得到3个杂交种CS71、CK1和CB1.以CMS3411-7、CS71、CK1和CB1为材料,以Polima为对照,利用多聚酶链式反应(Polymer-ase chain reaction,PCR)技术与田间实验育性观察相结合的方法,研究了orf224与几个芸薹属种间杂种雄性不育性之间的相关性.结果显示:在这4个材料中尽管均含有orf224基因,但CMS3411-7和CB1表现为雄性不育,CS71和CK1表现为雄性可育.这就表明,orf224虽是CMS的因子,但它必须和核基因进行互作才能发挥作用.

The correlation of the orf224 gene with cytoplasmic male sterility of several interspecific and inter-subspecific hybrids in the genus Brassica

orf224 is a mitochondrial gene discovered in Polima cytoplasm of rapeseed(Brassica napus),which is considered to be associated with cytoplasmic male sterility(CMS).CMS line CMS3411-7 of Chinese cabbage(B.campestris ssp.pekinensis)was produced by cross pollination and repeated backcrosses between Polima and Chinese cabbage inbred lines 3411-7 as recurrent male parent.Using CMS3411-7 as female parent,three F_1hybrids CS_71,CK_1and CB_1 were derived from the cross of CMS3411-7 with Pakchoi(B.campestris ssp.chinensis)inbred line S_71,Chinese cabbage inbred line K_1 and flowering Chinese cabbage(B.campestris ssp.chinensis var.utilis)inbred line B_1,respectively.In this paper,using CMS3411-7,CS_71,CK_1and CB_1as materials,Polima as control,the correlation of the orf224 gene with cytoplasmic male sterility of several interspecific and inter-subspecific hybrids in Brassica was studied by means of PCR and pollen fertility observation.The results showed that all the four materials contained orf224 gene,but CMS3411-7 and CB_1were male sterile,CS_71and CK_1were male fertile.These results indicated that orf224 was one of the factors of CMS,but orf224 must interact with nuclear gene to produce function.