| 菊花幼嫩花瓣愈伤组织的诱导和植株再生 |
| |
| 引用本文： | 司怀军,戴朝曦.菊花幼嫩花瓣愈伤组织的诱导和植株再生[J].甘肃农业大学学报,1998,33(2):175-177,174. |
| |
| 作者姓名： | 司怀军 戴朝曦 |
| |
| 作者单位： | 甘肃农业大学农业生物工程研究所 |
| |
| 摘 要： | 以菊花幼嫩花瓣作为供体材料，ＭＳ作为基本培养基，在激素组合为６ＢＡ１～３ｍｇ／Ｌ＋ＮＡＡ１ｍｇ／Ｌ或２，４ Ｄ１～３ｍｇ／Ｌ＋ＫＴ１ｍｇ／Ｌ的范围内均能诱导出愈伤组织，但以ＭＳ附加６ＢＡ２ｍｇ／Ｌ和ＮＡＡ１ｍｇ／Ｌ的诱导率最高，可达１００％。将愈伤组织转移到ＭＳ＋６ＢＡ２ｍｇ／Ｌ＋ＮＡＡ０２ｍｇ／Ｌ的培养基上，分化芽的频率为７７８％。将３ｃｍ左右的茎段转移到不含激素的ＭＳ培养基或ＭＳ＋ＮＡＡ０１ｍｇ／Ｌ的培养基上，生根率可达９５％以上。
|
| 关 键 词： | 菊花 花瓣 愈伤组织 植株再生 |
Callus Induction and Plant Regeneration of Young Chrysanthemum Petal |
| |
| Si Huaijun,Dai Chaoxi,Yu Pinhua,Wang Di,Wang Qing.Callus Induction and Plant Regeneration of Young Chrysanthemum Petal[J].Journal of Gansu Agricultural University,1998,33(2):175-177,174. |
| |
| Authors: | Si Huaijun Dai Chaoxi Yu Pinhua Wang Di Wang Qing |
| |
| Abstract: | Young petal of chrysanthemum was used as the donor tissue. callus was induced in MS media with hormone combination 6BA 1～3 mg/L + NAA 1 mg/L or 2,4 D 1～3 mg/L+KT 1 mg/L.The highest callus induction frequency (100 %) was obtained in the medium of MS supplemented with 6BA 2 mg/L and NAA 1 mg/L. After the callus was transferred to the regeneration medium (MS+6BA 2 mg/L+NAA 0 2 mg/L), the shoot regeneration frequency reached 77 8 %. In order to induce roots, the 3 cm shoots were transferred to the root induction media (MS+NAA 0 1 mg/L or hormone free), more than 95 % root induction frequency was obtained. |
| |
| Keywords: | chrysanthemum petal callus plant regeneration |
| 本文献已被 CNKI 维普 等数据库收录！ |
|
