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| 实验用封闭群金黄地鼠mtDNA D-环核苷酸组成与变异分析 | |
| 引用本文： | 贾晓蕊,宁磊,吴润,景志忠,王川,赵春林.实验用封闭群金黄地鼠mtDNA D-环核苷酸组成与变异分析[J].甘肃农业大学学报,2008,43(5). |
| 作者姓名： | 贾晓蕊 宁磊 吴润 景志忠 王川 赵春林 |
| 作者单位： | 1. 甘肃农业大学动物医学院,甘肃,兰州,730070 2. 兰州生物制品研究所,甘肃 兰州,730046 3. 中国农业科学院兰州兽医研究所,甘肃 兰州,730046 |
| 摘 要： | 对兰州、上海、北京和武汉4个封闭群共98只金黄地鼠线粒体DNA(mtDNA)D-环进行分析.结果表明:实验用封闭群金黄地鼠mtDNA D-环序列中,T、C、A、G碱基含量分别为30.9%2、5.2%、29.5%、14.4%,其中A+T为60.4%、G+C为39.6%,G+C含量明显低于A+T;发现的167个多态位点中,113个转换、52个颠换、2个位点既有转换也有颠换,分别占多态位点数的67.7%、31.1%1、.2%,没有插入/缺失同时出现的位点,表明我国实验用封闭群金黄地鼠个体间多态性丰富,群体间无明显变异. |
| 关 键 词： | 封闭群金黄地鼠 mtDNA D-环 遗传变异 |
Analysis of nucleotide content and variability of mtDNA D-loop sequence in laboratory closed colony of golden hamster |
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| JIA Xiao-rui,NING Lei,WU Run,JING Zhi-zhong,WANG Chuan,ZHAO Chun-lin.Analysis of nucleotide content and variability of mtDNA D-loop sequence in laboratory closed colony of golden hamster[J].Journal of Gansu Agricultural University,2008,43(5). | |
| Authors: | JIA Xiao-rui NING Lei WU Run JING Zhi-zhong WANG Chuan ZHAO Chun-lin |
| Abstract: | 98 golden hamsters from four laboratory closed colonies of Lanzhou,Shanghai,Beijing and Wuhan were used to analyze the mitochondrial DNA(mtDNA) D-loop.The results showed that the percentage of T,C,A,G in the mtDNA D-loop sequences of the laboratory closed colony golden hamster were 30.9 %,25.2 %,29.5 % and 14.4 % respectively,in which A+T was 60.4 %,G+C was 39.6 %,and the content of A+T was higher than G+C.Total of 167 polymorphism sites were found,in which 113 transition,52 transversion and 2 of both transition and transversion,the percentage of these sites were 67.66 %,31.14 % and 1.20 % respectively.However,there was no insertion/deletion.These indicated that Laboratory closed colony golden hamster in China has more rich of individual polymorphism and no-clear variation. |
| Keywords: | mtDNA |
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