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用改进的异硫氰酸胍一步法提取完熟水果RNA
引用本文:陆璐,李小娟.用改进的异硫氰酸胍一步法提取完熟水果RNA[J].甘肃农业大学学报,1999,34(2):112-115.
作者姓名:陆璐  李小娟
作者单位:[1]西北农业大学园艺系 [2]华南热带农业科学院国家热带生物技术重点实验室
基金项目:华南热带农业科学院热带生物技术重点实验开放研究基金
摘    要:以完熟水果为材料提取RNA,除了要最大限度地抑制内源RNA酶活性,严格杜绝外源RNA酶污染外,选择合适的试验材料也是试验成功的关键。选取健壮植株上发育正常的果实,剖取果皮或近果皮果肉组织,在Chomczynski硫氰酸肌一步法的基础上,进行了2次变性,反复抽提,无菌操作等技术改进,获得了满意的纯化效果。

关 键 词:RNA  异硫氰酸胍  水果

The Improved Single-Step Method to Isolate RNA from Ripening Fruit with Guanidinium Isothiocyanate
Lu Lu, Wang Ming.The Improved Single-Step Method to Isolate RNA from Ripening Fruit with Guanidinium Isothiocyanate[J].Journal of Gansu Agricultural University,1999,34(2):112-115.
Authors:Lu Lu  Wang Ming
Institution:Lu Lu; Wang Ming (of North-west Agricultural University of Horticulture of Department of Shanxi Yanglin 712100)Li Xiaojuan; ZhengXueqin (National Key BiotechnologyLaboratory for Tropical Crops Chinese Academy of Tropical Agriculture)
Abstract:To extract RNA from ripening fruit, besides with inhibiting activity of introRNase ultimately, refusing from contamination of extro-RNase, the choosing the suitable materials is the key point to be considre. The good resulbs can be obtained selecting normal developed fruit in vigorous living-plant, peeling scalp or flesh near scalp, based on Chomczynski's single-step method with guanidinium isothiocyanate, adopting twice-denature,repeating phenol-extraction and sterile operation, the good results can be obtained.
Keywords:RNA  guanidinium isothiocyanate  fruit
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