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| 幼龄小鼠小肠组织内参基因的选择 | |
| 引用本文： | 董晓丽,王加启,卜登攀,刘光磊,赵国琦,张春林,杨光,李旦,魏宏阳,周凌云.幼龄小鼠小肠组织内参基因的选择[J].甘肃农业大学学报,2009,44(5). |
| 作者姓名： | 董晓丽 王加启 卜登攀 刘光磊 赵国琦 张春林 杨光 李旦 魏宏阳 周凌云 |
| 作者单位： | 1. 中国农业科学院北京畜牧兽医研究所,动物营养学国家重点实验室,北京,100193;扬州大学动物科学与技术学院,江苏,扬州,225009 2. 中国农业科学院北京畜牧兽医研究所,动物营养学国家重点实验室,北京,100193 3. 扬州大学动物科学与技术学院,江苏,扬州,225009 |
| 基金项目： | 国家自然基金项目，动物营养国家重点实验室自主研究课题(2004DA125184 |
| 摘 要： | 选择B2 M、ACTB、GAPDH、SDHA、HPRT1和ARBP共6个内参基因,研究其在幼龄小鼠小肠组织内的表达情况.结果表明:6个内参基因均可获得特异性扩增产物;经geNorm程序和NormFinder分析,6个内参基因稳定度由高到低为SDHA=HRPT1>ARBP>ACTB>GAPDH>B2 M;基因表达的稳定值分别为0.006(SDHA)、0.006(HRPT1)、0.007(ARBP)、0.018(ACTB)、0.029(GAPDH)、0.111(B2 M),最终筛选出SDHA和HRPT1 2个内参基因适合用于校正目的基因的表达. |
| 关 键 词： | 实时定量PCR 内参基因 geNorm程序 Norm Finder程序 小鼠小肠组织 |
The selection of reference genes in young mouse small intestine |
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| DONG Xiao-li,WANG Jia-qi,BU Deng-pan,LIU Guang-lei,ZHAO Guo-qi,ZHANG Chun-lin,YANG Guang,LI Dan,WEI Hong-yang,ZHOU Ling-yun.The selection of reference genes in young mouse small intestine[J].Journal of Gansu Agricultural University,2009,44(5). | |
| Authors: | DONG Xiao-li WANG Jia-qi BU Deng-pan LIU Guang-lei ZHAO Guo-qi ZHANG Chun-lin YANG Guang LI Dan WEI Hong-yang ZHOU Ling-yun |
| Institution: | DONG Xiao-li1,2,WANG Jia-qi1,BU Deng-pan1,LIU Guang-lei1,ZHAO Guo-qi2,ZHANG Chun-lin1,YANG Guang1,LI Dan1,WEI Hong-yang1,ZHOU Ling-yun1(1.State Key Laboratory of Animal Nutrition,Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing 100193,China,2.College of Animal Science and Technology,Yangzhou University,Yangzhou 225009,China) |
| Abstract: | Reference genes are widely used for normalization of the expression level of mRNA in RT-PCR,but the expression of reference gene is influenced by physiological stage,tissues or cells,and the conditions of experiments.The purpose of the present study was to investigate the expression of B2M,ACTB,GAPDH,SDHA,HPRT1 and ARBP by RT-PCR in young mouse small intestine.By geNorm and normFinder analysis,SDHA and HPRT1 were finally determined as suitable reference genes and could be used to normalize mRNA levels betwe... |
| Keywords: | RT-PCR reference gene geNorm NormFinder small intestine |
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