蕙兰种子根状茎培养与愈伤组织诱导

以蕙兰种子无菌萌发的根状茎为材料，采用不同的激素配比以及不同的有机添加物，研究根状茎增殖和蕙兰愈伤组织诱导的适宜配方。研究结果表明:在蕙兰根状茎增殖的研究中添加100 g·L^-1香蕉泥在增殖率、分支数以及增加长度上均优于添加100 g·L^-1土豆泥；蕙兰根状茎出芽最佳的培养基:MS+2.0 mg·L^-1 NAA+4.0 mg·L^-1 6 BA+1.0 g·L^-1酪蛋白+1.0 g·L^-1蛋白胨+15 g·L^-1蔗糖+15 g·L^-1葡萄糖；诱导愈伤组织活力好并且诱导率高的培养基配方为MS+0.5 mg·L^-1 2,4 D + 1.0 mg·L^-1 NAA+1.0 mg·L^-1 6 BA+100 g·L^-1椰汁+30 g·L^-1 蔗糖。

Rhizome Culture and Callus Induction of Cymbidium faberi Rolfe Seeds

The rhizomes from Cymbidium faberi Rolfe seeds germinated aseptically were used as material to study the suitable formula of rhizome proliferation and Cymbidium faberi Rolfe callus induction with use of different hormone ratios and different organic additives.The results showed that in the study of rhizome proliferation of Cymbidium faberi Rolfe, addition of 100 g·L^-1 banana puree was better than addition of 100 g·L^-1 potato puree in proliferation rate, branch number and increasing length; the best medium for rhizome budding was MS+2.0 mg·L^-1 NAA + 4.0 mg·L^-1 6 BA + 1.0 g·L^-1 casein + 1.0 g·L^-1 peptone + 15 g·L^-1 sucrose + 15 g·L^-1 glucose,the best medium for callus induction was MS + 0.5 mg·L^-1 2,4 D + 1.0 mg·L^-1 NAA + 1.0 mg·L^-1 6 BA + 100 g·L^-1 coconut juice + 30 g·L^-1 sucrose. ;