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| 基于ITS序列分析探讨大兴安岭地区野生黑木耳菌株的遗传多样性 | |
| 引用本文： | 刘华晶,许修宏,姜廷波,邹学忠.基于ITS序列分析探讨大兴安岭地区野生黑木耳菌株的遗传多样性[J].四川农业大学学报,2011,29(1). |
| 作者姓名： | 刘华晶 许修宏 姜廷波 邹学忠 |
| 作者单位： | 1. 东北农业大学资源与环境学院,哈尔滨,150030;东北林业大学林学院,哈尔滨,150040 2. 东北农业大学资源与环境学院,哈尔滨,150030 3. 东北林业大学林学院,哈尔滨,150040 |
| 基金项目： | 科技部十一五科技支撑项目，科技部十一五科技支撑项目，东北农业大学资源与环境学院青年基金，东北农业大学创新团队发展计划 |
| 摘 要： | 通过ITS序列对大兴安岭地区采集的14株野生黑木耳菌株和6株对照栽培种黑木耳菌株进行亲缘关系分析,以揭示不同菌株之间的遗传关系。利用软件Clustal X 1.83和MAGA 4.1进行系统发育分析。结果表明:①黑木耳ITS区(ITS1+5.8S+ITS2)信息位点比例达到52.1%,遗传位点丰富,证明ITS序列可以为黑木耳菌株的亲缘关系问题提供较强的证据。②在MP系统树中,黑木耳菌株明显聚为2类,聚类结果表明黑木耳野生菌株间遗传多样性优于栽培菌株。 |
| 关 键 词： | 黑木耳 ITS 遗传多样性 |
A Study on Genetic Diversity of wild Auricularia auricula in Greater Khingan Mountains Area Based on ITS Sequence Analysis |
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| LIU Hua-jing,XU Xiu-hong,JIANG Ting-bo,ZOU Xue-zhong.A Study on Genetic Diversity of wild Auricularia auricula in Greater Khingan Mountains Area Based on ITS Sequence Analysis[J].Journal of Sichuan Agricultural University,2011,29(1). | |
| Authors: | LIU Hua-jing XU Xiu-hong JIANG Ting-bo ZOU Xue-zhong |
| Abstract: | Genetic relationship among different strains was disclosed by analyzing the internal transcribed spacer(ITS) sequences of 14 wild Auricularia auricula strains and 6 cultivated strains from Greater Khingan Mountains area.The software ClustalX 1.83 and MEGA 4.1 were used to conduct the phylogeny analysis.The results were as follows:the information sites of ITS Region(ITS1+5.8S+ITS2) reached 52.1%,which proved that ITS sequence could provide strong evidences for the genetic relationship among Auricularia auricular strains.② in MP trees,Auricularia auricula strains were obviously divided into 2 groups,and there were more genetic diversities in wild strains than in cultivated strains. |
| Keywords: | ITS |
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