| 西洋杜鹃组织培养技术体系研究(Ⅰ)——基本培养基和外植体的选择 |
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| 引用本文： | 钟宇,张健,罗承德,陈其兵.西洋杜鹃组织培养技术体系研究(Ⅰ)——基本培养基和外植体的选择[J].四川农业大学学报,2001,19(1):37-39. |
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| 作者姓名： | 钟宇 张健 罗承德 陈其兵 |
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| 作者单位： | 四川农业大学林学园艺学院, |
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| 摘 要： | 实验以不同季节的茎尖 (含茎段 )为材料 ,采用不同基本培养基 ,研究确定西洋杜鹃组织培养适合的基本培养基类型 ,植株再生的可能途径 ,以及适宜的培养条件。结果表明 :①低盐分浓度及高比值NH4 +/NO3 -的基本培养基Read培养基适合西洋杜鹃 ;②最适外植体为摘花芽后萌发的顶芽茎尖 ;③最佳取材时间为 3～ 5月 ;④培养条件 :温度 2 5± 2℃ ,光照 16h/黑暗 8h ,光照强度 15 0 0～ 2 5 0 0lx。
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| 关 键 词： | 西洋杜鹃 组织培养 快速繁殖 |
| 文章编号： | 1000-2650(2001)01-0038-03 |
| 修稿时间： | 2001年2月15日 |
A Study on the Technique
System of Tissue Culture in Rhododendron Hybrind( |
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| ZHONG Yu,ZHANG Jian,LUO Cheng-de,CHEN Qi-bing.A Study on the Technique
System of Tissue Culture in Rhododendron Hybrind([J].Journal of Sichuan Agricultural University,2001,19(1):37-39. |
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| Authors: | ZHONG Yu ZHANG Jian LUO Cheng-de CHEN Qi-bing |
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| Abstract: | In view of studying the suitable type of medium, probable way of explant regeneration and suitable condition of culture for tissue culture in R.hybridn, a experimentation was established in which shoot tip from different seasons and a series of different medium were employed. The results are as follows: ①Read medium with low mineral salt concentration and high ration of NH4+/NO3- is suitable for R.hybridn microculture; ②The optimal explants are shoot tips of end buds excised from R.hybridn in 3～5 months; ③All the cultures are kept under the temperature of 25±2℃, illuminated for 16 hours per day, and light intensity was 1500～2500lx. |
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| Keywords: | R HYBRIDN TISSUE CULTURE RAPID PROPAGATION |
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