| 产纤维素酶枯草芽孢杆菌C-36的产酶条件研究 |
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| 引用本文： | 韩学易,陈惠,吴琦,梁如玉,高凤菊,胥兵.产纤维素酶枯草芽孢杆菌C-36的产酶条件研究[J].四川农业大学学报,2006,24(2):178-181. |
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| 作者姓名： | 韩学易 陈惠 吴琦 梁如玉 高凤菊 胥兵 |
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| 作者单位： | 1. 四川农业大学,生命科学与理学院,四川,雅安,625014
2. 四川农业大学,资源与环境学院,四川,雅安,625014 |
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| 摘 要： | 以产纤维素酶的枯草芽孢杆菌菌株C-36为研究对象,从碳源、氮源、接种量、培养基初始pH、温度等方面研究该菌株的产酶条件,结果表明该菌产酶的最适碳源为2%的CMC-Na,最适氮源为2.5%的蛋白胨+酵母粉复合氮源,最佳接种量为4%,最适起始pH为5.0,产酶最适温度为37℃。在此条件下,培养36 h后达到产酶高峰,CMC酶活为196.33 U/mL,是优化前的3倍。
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| 关 键 词： | 纤维素酶 枯草芽孢杆菌 产酶条件 |
| 文章编号： | 1000-2650（2006）02-0178-04 |
| 收稿时间： | 2005-09-29 |
| 修稿时间： | 2005年9月29日 |
Optization of Conditions for Submerged Fermentation of Cellulase from Bacillus subtilis C-36 |
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| HAN Xue-yi,CHEN Hui,WU Qi,LIANG Ru-yu,GAO Feng-ju,XU Bing.Optization of Conditions for Submerged Fermentation of Cellulase from Bacillus subtilis C-36[J].Journal of Sichuan Agricultural University,2006,24(2):178-181. |
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| Authors: | HAN Xue-yi CHEN Hui WU Qi LIANG Ru-yu GAO Feng-ju XU Bing |
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| Institution: | 1. College of Biology and Science; 2. College of Resource and Environment, Sichuan Agricultural University, Yaan 625014, Siehuan, China |
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| Abstract: | Study on the optization of condition for submerged fermentation of strain Bacillus subtilis cellulase was made: the optimum carbon source and nitrogen source is 2% CMC-Na,2.5% peptone and yeast extract;the optimum quantity of inoculation is 4%;when the optimum pH is 5.0;the optimum temperature is 37?℃;it arrives the zymogenous peak at 36?h,and the CMCase activity is 196.33?U/mL which is three times of the previous. |
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| Keywords: | cellulase Bacillus subtilis fermentation condition |
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