小麦高代材料中幼穗培养特性的研究

研究分析了两个小麦杂交组合的F5株系群体 ,其中组合 1包含了较高组织培养能力的亲本 ,组合 2的亲本均有较低的组织培养能力。研究结果显示 ,两个组合F5群体的半愈期、成愈率 ,成根愈率和成芽愈率的分布显著不同。组合 1的F5群体中有显著较多的具有高效组织培养能力的株系。研究结果指出 ,即使在分离世代未加选择的情况下 ,高效组织培养能力有关的基因仍然能够遗传到F5世代。因此 ,在F5代株系中 ,结合农艺性状的选择 ,培育农艺性状优良、组织培养表现高效的小麦株基因受体品系是可能的

Study on Tissue Culture Response in Wheat (Triticuma estivum L.) F5 Lines

The wheat F5 lines originated from different combinations were analyzed for determining their tissue culture capacity. The combination 1 contains two parents with excellent ability in tissue culture, but combination 2 does not. More significant lines, which exhibited excellent characters in tissue culture, such as the days of calluing at 50%, the rate of total calli induction, the rate of root formation, the rate of shoot information, were observed in combination 1 than combination 2. The results indicate that the F5 lines contain the genes of tissue culture characters from their wheat parents, in despite of selection lack in F2～F4 for tissue culture characters. In wheat breeding for transgenic receptors it is an approach to select tissue culture abilities with excellent agronomic characteristics in F5 plant lines.