不同环境条件下水稻株高的QTL定位分析

用水稻测序品种培矮64s和Nipponbare为亲本构建的含137个SSR标记的连锁遗传图谱和(培矮64s/Nipponbare)F2、F2∶3群体的180个单株(株系)对水稻的株高性状进行了2年2点的QTL定位分析。2年2点共检测到8个QTL分别位于第1、2、3、4、5、7、10染色体,表型贡献率6.9%～47.7%。F2群体(成都试点)共检测到6个QTL,分布在第1、1、3、4、5、7染色体上,F2∶3群体(海南试点)共检测到4个QTL,分布在第1、2、4、10染色体上,其中位于第1、4染色体上的qPH1-2和qPH4为重复检测到的QTL。对所定位QTL的价值、用QTL定位预测基因的功能等进行了探讨。

Mapping of Quantitative Traits Loci for Plant Height of Rice under Different Environments

Quantitative trait loci (QTL) for plant height of rice were mapped under two envirorments in 2003 and 2004 by means of F_2 and F_ 2∶3 populations consisted of 180 plants (lines) from the cross between two genome sequenced rice varieties “Peiai64s” and “Nipponbare”, and a genetic linkage map including 137 SSR markers constituted by (Peiai 64s/Nipponbare) F_2 population. A total of eight QTLs for plant height were identified in chromosmesJP2] 1, 2, 3, 4, 5, 7 and 10, with 6.9%～47.7% of phenotypic percentage of contribution; Six QTLs were identified in chromosomes 1, 1, 3, 4, 5 and 7 in F_2 population in Chengdu, and four QTLs in chromosomes 1, 2, 4 and 10 in F_ 2∶3 population in Hainan. In these QTLs, two QTLs qPH1-2WTBZ] and qPH4WTBZ] located in chromosomes 1 and 4 were repeatedly identified. Finally, the worth of mapping QTLs and prediction of gene function by QTL mapping etc. were discussed.