| 2,4D、KT对小麦成熟胚愈伤组织形成、分化的影响 |
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| 引用本文： | 唐宗祥,张怀琼,张怀渝,晏本菊,任正隆.2,4D、KT对小麦成熟胚愈伤组织形成、分化的影响[J].四川农业大学学报,2004,22(3):203-205. |
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| 作者姓名： | 唐宗祥 张怀琼 张怀渝 晏本菊 任正隆 |
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| 作者单位： | 四川农业大学,植物遗传育种省级重点实验室,四川,雅安,625014 |
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| 基金项目： | 四川省科技厅生物技术攻关项目. |
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| 摘 要： | 以小麦新品种川农 17、12成熟胚为外植体进行离体培养 ,结果表明 :2mg/L 2 ,4 D有利于胚性愈伤组织形成 ,5mg/LKT有利于植株再生 (两品种植株再生率分别为 73 6 3% ,89 4 7% )。高浓度 2 ,4 D(4～ 8mg/L)虽不影响成愈率 ,但抑制植株再生。这种抑制作用能被KT拮抗。而KT的这种拮抗作用在 2mg/L 2 ,4 D条件下不能体现。
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| 关 键 词： | 小麦 成熟胚 植株再生 植物激素 |
| 文章编号： | 1000-2650(2004)03-0203-03 |
| 修稿时间： | 2004年6月8日 |
Effect of 2,4-D,KT on Callus Formation and Plantlet Regeneration from Mature Wheat (Triticum aestivum L.) Embryos |
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| TANG Zong-xiang,ZHANG Huai-qiong,ZHANG Huai-yu,YAN Ben-ju,REN Zheng-Long.Effect of 2,4-D,KT on Callus Formation and Plantlet Regeneration from Mature Wheat (Triticum aestivum L.) Embryos[J].Journal of Sichuan Agricultural University,2004,22(3):203-205. |
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| Authors: | TANG Zong-xiang ZHANG Huai-qiong ZHANG Huai-yu YAN Ben-ju REN Zheng-Long |
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| Institution: | TANG Zong-xiang,ZHANG Huai-qiong,ZHANG Huai-yu,YAN Ben-ju,REN Zheng-Long~ |
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| Abstract: | Mature wheat embryos of new wheat varieties Chuannong 17 and 12 were cultured in vitro. The results show that 2?mg/L 2,4-D is beneficial to the formation of totipotent calli. 5?mg/L KT is beneficial to plant regeneration (The regeneration frequencies of the two cultivars are 73.63%, 89.74% respectively). High concentration of 2,4-D (4～8?mg/L) has no bad effect on callus formation, but they inhibit plant regeneration. This kind of inhibition is resisted by KT, but in the initiation media supplemented with 2?mg/L 2,4-D this kind of resistance disappears. |
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| Keywords: | wheat mature embryo plant regeneration phytohormone |
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