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红叶乌桕茎段离体培养的研究
引用本文:韩珊,石大兴,王米力,史伟,张先.红叶乌桕茎段离体培养的研究[J].四川农业大学学报,2006,24(3):300-302.
作者姓名:韩珊  石大兴  王米力  史伟  张先
作者单位:1. 四川农业大学,林学园艺学院,四川,雅安,625014
2. 成都农业科技职业学院,四川,成都,611130
基金项目:四川省重点学科建设项目
摘    要:以带芽茎段、茎尖做外植体,建立了红叶乌桕的组织培养和再生体系。结果表明:①外植体用升汞分两次共处理8 min,污染率低;②启动培养以MS+6-BA 1.0 mg/L+2,4-D 0.05 mg/L+NAA 0.1 mg/L为最佳配方;③接种后先进行暗培养1周,启动较快;④继代培养以MS+6-BA 1.0 mg/L+IBA 0.1 mg/L,其月增殖系数为6.17;⑤生根诱导以1/2MS+NAA 1.0 mg/L+IBA 0.1 mg/L效果最好,其生根率为87.6%;⑥生根苗移栽到珍珠岩∶蛭石∶河沙=3∶2∶1的育苗基质中,成活率为83.2%。

关 键 词:红叶乌桕  茎段及茎尖  离体培养
文章编号:1000-2650(2006)03-0300-03
收稿时间:2005-12-15
修稿时间:2005年12月15

Study on Shoots Culture in Vitro of Euphorbia cotinifolia Linn
HAN Shan,SHI Da-xing,WANG Mi-li,SHI Wei,ZHANG Xian.Study on Shoots Culture in Vitro of Euphorbia cotinifolia Linn[J].Journal of Sichuan Agricultural University,2006,24(3):300-302.
Authors:HAN Shan  SHI Da-xing  WANG Mi-li  SHI Wei  ZHANG Xian
Institution:1.College of Forestry and Horticulture, Sichuan Agricultural University, Yaan 625014, Sichuan, China; 2. Chengdu Agricultural Science and Technology Vocational College, Chendu 611130, China
Abstract:The explants from Euphorbia cotinifolia(L.) stem fragments and shoot tips were used to establish culture in vitro and regeneration system.The results show that: ①The explants are sterilized for 8 min with 0.1% HgCl_2 in two times.It can reduce the infection rate;②Initiation medium: MS+6-BA 1.0 mg/L+2,4-D 0.05 mg/L+NAA 0.1 mg/L is the best one;③The shoots cultured in dark can get good effect for one week;④Subculture medium: MS+6-BA 1.0 mg/L+IBA 0.1 mg/L;The increasing coefficient in a month was 6.17; ⑤Rooting medium: 1/2MS+NAA 1.0 mg/L+IBA 0.1 mg/L give best results for rooting;⑥The rooted plantlets are transferred into the substrate(pearlite∶vermiculite∶sand=3∶2∶1),and the survival rate is 83.2%.
Keywords:Euphorbia cotiniflia Linn    stem fragments and shoot tips  vitro culture
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