产纤维素酶芽孢杆菌C-36的分离筛选及其鉴定

以羧甲基纤维素钠为唯一碳源,从废泥浆中经反复筛选及刚果红鉴定,获得一株高活力纤维素酶生产菌株C-36。与枯草芽孢杆菌标准菌株进行了参比试验,初步鉴定该细菌为枯草芽孢杆菌。生长条件的测定显示该菌生长pH范围较宽,在pH 7.0生长最好,在pH 8.0~10.0的碱性条件下长势较好。摇瓶发酵粗酶液的性质测定表明,该菌所产生的内切酶在pH 7.0左右酶活较高,酶活力为1.280 IU/mL。

Selection and Identification of Cellulase-producing Bacillus C-36

One strain of cellulase-producing bacteria which can degrade sodium carboxymethylcellulose was isolated on the selected medium by using CMC-Na as only carbon source from the mud.The strain has high endo-β-glucanase producing ability proved by the congo red dying method on the culture plates.It is identified as Bacillus subilius according to its morphological,physiological and biochemiscal characteristics.It is revealed by the experiments in which C-36 grows in a broad range of pH.While the optimum pH for its growing is pH 7.0 and it also grows well in more alkaline circumstances.Results in shaking flask culture show that high cellulase activity(CMCase) can be detected in the condition of pH 7.0 and the cellulase activity is 1.280?IU/mL.