| 一种适用于甘蔗SSR-PCR的DNA快速提取方法 |
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| 引用本文： | 陈忠良,秦翠鲜,郭元元,杨翠芳,罗海斌,何海波.一种适用于甘蔗SSR-PCR的DNA快速提取方法[J].广西农业科学,2010,41(11):1155-1157. |
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| 作者姓名： | 陈忠良 秦翠鲜 郭元元 杨翠芳 罗海斌 何海波 |
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| 作者单位： | 广西作物遗传改良生物技术重点开放实验室;农业部甘蔗品质监督检验测试中心(南宁);广西甘蔗遗传改良重点实验室;广西师范大学生命科学学院; |
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| 基金项目： | 国家科技支撑计划项目，广西自然科学基金，广西科学研究与技术开发计划项目，广西农业科学院基本科研业务专项项目 |
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| 摘 要： | 以不同甘蔗品种幼苗期幼嫩叶片为材料,以改良SDS法和DNA快速提取法对甘蔗基因组DNA进行提取,并以SSR-PCR检测比较两者的扩增效果。结果表明,两种方法提取获得的基因组DNA条带基本一致,条带较为清晰,重复性好且稳定,均可满足SSR-PCR扩增的需要。但DNA快速提取法简化了SDS改良法,具有高通量、操作方便、简单、快速、成本低、DNA存放时间较长等优点,能在较短时间内完成大量样品的DNA提取。
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| 关 键 词： | 甘蔗 DNA提取 SDS改良法 DNA快速提取法 |
A rapid DNA extraction method from sugarcane leaves for SSR-PCR |
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| CHEN Zhong-liang,QIN Cui-xian,GUO Yuan-yuan,YANG Cui-fang,LUO Hai-bin,HE Hai-bo.A rapid DNA extraction method from sugarcane leaves for SSR-PCR[J].Guangxi Agricultural Sciences,2010,41(11):1155-1157. |
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| Authors: | CHEN Zhong-liang QIN Cui-xian GUO Yuan-yuan YANG Cui-fang LUO Hai-bin HE Hai-bo |
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| Institution: | CHEN Zhong-liang1,2,QIN Cui-xian1,3,GUO Yuan-yuan1,YANG Cui-fang1,LUO Hai-bin1,HE Hai-bo1,3(1 Guangxi Crop Genetic Improvement and Biotechnology Lab,Nanning 530007,China,2 Sugarcane Quality Inspection and Test Center in Nanning,Ministry of Agriculture/Guangxi key Laboratory of Sugarcane Genetic Improvement,3 College of Life Sciences,Guangxi Normal University,Guilin,Guangxi 541004,China) |
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| Abstract: | The young leaves of different sugarcane varieties were sampled at settling stage to extract genomic DNA,and a rapid DNA extraction method was developed by modifications in SDS method.The extracted DNA was amplified in PCR using SSR primers.The results showed no differences in the amplified bands of the DNA isolated using the two different methods.The DNA isolated from rapid method showed clear and reproducible bands,and was found suitable for the application in marker related studies.Further,the DNA isolate... |
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| Keywords: | sugarcane DNA extraction SDS modified method rapid DNA extraction |
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