SCoT分子标记技术在丝瓜上的应用

【目的】采用SCo T分子标记技术分析丝瓜种质资源的遗传多样性和亲缘关系,为丝瓜种质资源的利用和品种选育提供参考。【方法】采用80条引物和SCo T-PCR对81份丝瓜种质资源进行遗传多样性分析和聚类分析。【结果】在优化的SCo T-PCR反应体系基础上,从80条引物中筛选获得10条多态性丰富、重复性好的引物,每条引物扩增获得10-17条100-2100 bp的条带;10条引物共扩增获得140条带,其中多态性条带124条,多态性比例87.57%。81份丝瓜种质材料的遗传相似系数在0.5900-0.9200,在0.5900处可将所有供试材料划分为两大类,第Ⅰ类包括46份有棱丝瓜材料,第Ⅱ类包括35份普通丝瓜材料,广西和广东的丝瓜种质材料主要聚在第Ⅰ类。在遗传相似系数0.7200和0.7900处,第Ⅰ、Ⅱ大类丝瓜材料均可划分为5个组,每组丝瓜材料主要来自相同地区。【结论】SCo T分子标记可作为ISSR、SRAP等标记的有效补充,为丝瓜种质资源的研究提供简单、可靠的工具。

Application of SCoT markers on genetic diversity analysis of Luffa Mill.

【Objective】The genetic diversity and relationship of Luffa Mill. Germplasm resources were analyzed by using Sco T marker technology in order to provide references for resources utilization and variety breeding of loofah.【Method 】The genetic diversity and cluster analysis of 81 loofah accessions were conducted by using 80 primers and Sco T-PCR. 【Result 】Based on the optimal reaction system of Sco T-PCR, 10 primers with abundant polymorphism and better repeatability were screened from 80 primers, and each primer could amplify out 10-17 bands with 100-2100 bp in length. A total of 140 bands were amplified, and 124 of which were polymorphic, the polymorphic rate was87.57%. The genetic similar coefficient of 81 loofah accessions ranged from 0.5900 to 0.9200, and all loofah accessions could be clustered into 2 groups at 0.5900 of similar coefficients. The Ⅰ group included 46 accessions of Luffa acutangula（ L1） Roxb, which mainly contained most of loofah materials collected from Guangxi and Guangdong province. TheⅡ group included 35 accessions of Luffacy lindrica（L1） Roem. The loofah materials in Ⅰ and Ⅱ groups could be divided into 5 subgroups at 0.7200 and 0.7900 of similar coefficient, and each subgroup mainly contained the materials collected from the same region. 【Conclusion 】Sco T marker technology could be acted as effective supplement of ISSR and SRAP markers and provide a simple, reliable method on studying loofah germplasm resources.