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| 紫外分光光度法测定芒果叶提取物中芒果苷的含量 | |
| 引用本文： | 谢梅冬,邹步珍,邹煜,钟旭倩.紫外分光光度法测定芒果叶提取物中芒果苷的含量[J].广西农业科学,2010,41(9):968-970. |
| 作者姓名： | 谢梅冬 邹步珍 邹煜 钟旭倩 |
| 作者单位： | 1. 广西兽药监察所,南宁,530001 2. 南宁市百济生物工程有限公司,南宁,530001 3. 广西北海进出境检验检疫局,广西北海,536000 |
| 基金项目： | 科技型中小企业技术创新基金项目 |
| 摘 要： | 以50%乙醇为溶剂,采用紫外分光光度法测定芒果叶提取物中芒果苷的含量。结果表明,芒果苷在319nm波长处有最大吸收峰,且峰形一致,无杂峰干扰;经线性关系考察,在5～50μg/mL范围内线性关系良好（R^2=0.9968）;检测过程稳定性在2h以内。与HPLC法比较,该方法具有快速、简便、准确、重现性好等优点,可用于实际生产中芒果苷含量测定。 |
| 关 键 词： | 紫外分光光度法 芒果苷 含量测定 |
Determination of mangiferin content in extract of mango leaves by UV-spectrophotometry |
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| XIE Mei-dong,ZOU Bu-zhen,ZOU Yu,ZHONG Xu-qian.Determination of mangiferin content in extract of mango leaves by UV-spectrophotometry[J].Guangxi Agricultural Sciences,2010,41(9):968-970. | |
| Authors: | XIE Mei-dong ZOU Bu-zhen ZOU Yu ZHONG Xu-qian |
| Institution: | 1 Guangxi Institute of Veterinary Drugs Supervision,Nanning 530001,China;2 Baiji Biological Engineering Co.Ltd.of Nanning City,Nanning,Guangxi 530001,China;3 Beihai Entry-Exit Inspection and Quarantine Bureau of Guangxi,Beihai,Guangxi 536000,China) |
| Abstract: | Ultraviolet spectrophotometry was used to determine the mangiferin content in alcohol（50%） extract of mango leaves.The maximum absorbance of mangiferin was recorded at the wavelength of 319 nm,without any other peaks.In the range of 5-50 μg/mL,the linear relation of the linear equation was found significant（R^2=0.9968）.The stable time of mangiferin determination was 2 h.As compared to HPLC method,the method used in this research was simple,quick,accurate,reliable and reproducible,and could be used to determine the mangiferin content in mango leaf extract. |
| Keywords: | UV-spectrophotometry mangiferin content determination |
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