广西桉蝙蛾病原真菌的鉴定

【目的】进行广西桉蝙蛾病原真菌的种类鉴定,筛选致病性强的优势菌株,为桉蝙蛾的生物防治奠定基础。【方法】采用常规的组织分离法进行病原菌的分离,对分离纯化获得的菌株进行致病性测定,根据形态特征和rDNA ITS序列分析确定病原菌的分类地位。【结果】从桉蝙蛾虫尸上分离纯化获得206个真菌菌株,这些菌株分属于白僵菌（Beauveria）、镰刀菌（Fusarium）、拟青霉（Paecilomyces）、曲霉（Aspergillus）、青霉（Penicillium）、毛霉（Mucor）和Evlachovaea,其中白僵菌分离率为87.4%,其他真菌分离率较低。经致病性测定,白僵菌是桉蝙蛾幼虫强致病菌,镰刀菌和Evlachovaea是弱致病菌,其他4类真菌为非致病菌。以形态学特征和rDNA ITS序列分析结果为依据,鉴定球孢白僵菌B.bassiana（Balsamo）Vuillemin]、尖孢镰刀菌（F.oxysporum Schlecht）和Evlachovaea sp.为桉蝙蛾幼虫的致病菌,其中Evlachovaea sp.在中国首次被记载。【结论】广西桉蝙蛾的病原真菌为球孢白僵菌、尖孢镰刀菌和Evlachovaea sp.,其中球孢白僵菌为优势菌,具有较高的开发利用价值。

Identification of entomopathogenic fungi from Endoclyta signifer in Guangxi

【Objective】The resources of entomopathogenic fungi from Endoclyta signifer were identified to screen out the dominant isolates with strong pathogenicity and provide references for biological control of Endoclyta signifer.【Method】Entomopathogenic fungi of E. signifer were isolated by common tissue isolation method. The pathogenicity test of acquired fungi against E. signifer was conducted and taxonomic position of entomopathogenic fungi was determined by morphological characteristics and rDNA ITS sequence. 【Result】A total of 206 fungal isolates were obtained from the diseased E. signifer.They were identified as genus of Beauveria,Fusarium,Paecilomyces,Aspergillus,Penicillium,Mucor and Evlachovaea,among which isolation rate of Beauveria was 87.4% and isolation rates of other fungi were relatively low. After pathogenicity tests were completed, it showed that Beauveria bassiana was the predominant species with stronger pathogenicity. Fusarium and Evlachovaea were weakly pathogenic fungi and others were non-pathogenic fungi. B. bassiana, F. oxysporum and Evlachovaea sp. were identified by morphological characteristics and rDNA ITS sequences. Evlachovaea sp. was recorded in China for the first time. 【Conclusion】Entomopathogenic fungi of Endoclyta signifer in Guangxi are B. bassiana, F. oxysporum and Evlachovaea sp, of which B. bassiana is the predominant species with high application value.