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宁波地区南美白对虾桃拉综合症病毒(TSV)的检测与分析
引用本文:王建平,余晓巍,顾建明,吴雄飞.宁波地区南美白对虾桃拉综合症病毒(TSV)的检测与分析[J].广西农业科学,2009,40(9):1239-1242.
作者姓名:王建平  余晓巍  顾建明  吴雄飞
作者单位:1. 宁波市海洋与渔业研究院,浙江宁波,315012
2. 宁波大学生命科学与生物工程学院,浙江宁波,315211
3. 象山县新桥镇人民政府,浙江象山,315726
基金项目:国家科技支撑计划项目,宁波市重大科技攻关项目 
摘    要:桃拉综合症病毒(Taura Syndrome Virus,TSV)是对虾养殖业危害较严重的病毒之一。应用RT—PCR技术,对2004~2008年在宁波地区收集的1201份南美白对虾样品进行TSV检测,旨在调查了解该地区TSV的流行情况,为科学防控该病的发生提供参考。结果表明,被检样品中,44份样品呈现阳性结果,总阳性率为3.66%,且2004~2008年南美自对虾TSV阳性检出率呈逐年降低的趋势。可见,宁波地区南美白对虾桃拉综合症已得到有效防制。

关 键 词:南美白对虾  桃拉综合症病毒(TSV)  RT-PCR  检测

Detection and analysis of taura syndrome virus in Penaeus vannamei in Ningbo inshore
WANG Jian-ping,YU Xiao-wei,GU Jian-ming,WU Xiong-fei.Detection and analysis of taura syndrome virus in Penaeus vannamei in Ningbo inshore[J].Guangxi Agricultural Sciences,2009,40(9):1239-1242.
Authors:WANG Jian-ping  YU Xiao-wei  GU Jian-ming  WU Xiong-fei
Institution:WANG Jian-ping, YU Xiao-wei, GU Jian-ming, WU Xiong-fei (1 Ningbo Academy of Ocean and Fishery, Ningbo, Zhejiang 315012, China; 2 Faculty of Life Science and Biotechnology, Ningbo University, Ningbo, Zhejiang 315211, China ; 3 Rural Economic Office of the People' s Government of Xinqiao Town, Xiangshan County, Xiangshan, Zhejiang 315726, China)
Abstract:Taura syndrome virus (TSV) is one of the important viruses that affect Penaeus vannami severely and interferes with the breeding programs. In the present study, 1201 Penaeus vannami samples were collected from Ningbo inshore from 2004 to 2008. All the samples were analyzed for the detection of TSV using RT-PCR to work out the prevalent situation of TSV and provide the suggestion for control and prevention of TSV incidence. Out of 1201 samples analyzed, 44 samples were found to be positive with rate of 3.36%. It was also observed that the occurrence of TSV in samples decreased continuously from 2004 to 2008 due to the control measures applied showing the effectiveness of control measures on tanra syndrome of Penaeus vannami in Ningbo inshore.
Keywords:RT-PCR
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