| 杨梅树皮提取物中总黄酮快速分析方法研究 |
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| 引用本文： | 董悦涵.杨梅树皮提取物中总黄酮快速分析方法研究[J].广西农业科学,2011(10):1263-1266. |
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| 作者姓名： | 董悦涵 |
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| 作者单位： | 浙江经贸职业技术学院应用工程系; |
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| 基金项目： | 浙江经贸职业技术学院科研项目(09QN05);浙江经贸职业技术学院大学生科技创新创业项目(2008003); 浙江工业大学重中之重学科开放研究基金项目(2007) |
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| 摘 要： | 【目的】建立杨梅树皮提取物中总黄酮含量的快速分析方法。【方法】以芦丁为标准品,利用紫外分光光度法对杨梅树皮提取物中总黄酮类物质进行测定。【结果】用紫外分光光度法测定时,在其最大吸收峰417nm处测得标准品在0～20.40μg/mL的浓度范围内线性关系良好（R2=0.9996）,平均回收率为99.7%,RSD=1.69%。【结论】以AlCl3作显色剂的紫外分光光度法测定杨梅树皮提取物中黄酮类物质重复性、精密度及稳定性良好,回收率高,且操作简便,可作为杨梅树皮总黄酮提取工艺质量控制的一种检测手段。
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| 关 键 词： | 杨梅 树皮提取物 总黄酮 紫外分光光度法 |
Development of method for quick analysis of flavonoids in bark extract of Myrica rubra |
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| DONG Yue-han.Development of method for quick analysis of flavonoids in bark extract of Myrica rubra[J].Guangxi Agricultural Sciences,2011(10):1263-1266. |
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| Authors: | DONG Yue-han |
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| Institution: | DONG Yue-han(Department of Applied Engineering,Zhejiang Economic & Trade Polytechnic,Hangzhou 310018,China) |
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| Abstract: | Objective]A method for quick analysis of flavonoids in the bark extract of Myrica rubra was developed.Method]The flavonoid contents in the bark extract of Myrica rubra were determined by UV spectrophotometry using Rutin as standard sample.Result]The linear relation of the control（standard sample） was within the range of 0-20.4 μg/mL at the maximum wavelength of 417 nm（R2=0.9996） .The average recovery was 99.7% and the RSD was 1.69%.Conclusion]The developed method was simple,accurate,stable and had good reproducibility for analyzing flavonoids using AlCl3 as coloring agent and may be used to determine the flavonoids in the bark extract of Myrica rubra. |
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| Keywords: | Myrica rubra bark extract flavonoids UV spectrophotometry |
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