| 壳寡糖诱导甘蔗叶多酚与防御酶活性的变化 |
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| 引用本文： | 周桂,靳晓芸,邓光辉,丘立杭,邹成林,李杨瑞.壳寡糖诱导甘蔗叶多酚与防御酶活性的变化[J].广西农业科学,2011(8):874-877. |
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| 作者姓名： | 周桂 靳晓芸 邓光辉 丘立杭 邹成林 李杨瑞 |
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| 作者单位： | 广西民族大学;广西亚热带生物资源保护利用重点实验室;广西大学农学院;中国农业科学院甘蔗研究中心/广西农业科学院甘蔗研究所/广西甘蔗遗传改良重点实验室;广西作物遗传改良生物技术重点开放实验室; |
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| 基金项目： | 国家科技支撑计划项目(2007BAD30B03); 广西科技攻关重点项目(桂科攻0782004-3);广西科技攻关项目(桂科能0815011); 广西自然科学基金项目(2010GXNSFA013101); 广西教育厅项目(200707MS034); 广西民族大学引进人才项目(2010QD022) |
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| 摘 要： | 【目的】探讨壳寡糖对甘蔗抗性相关次生代谢物及相关防御酶的影响。【方法】以甘蔗品种新台糖22号（ROC22）为材料,拔节初期对甘蔗叶片分别喷施25、50和75mg/L壳寡糖溶液,以喷施清水为对照（CK）。喷施1、2、3、4d后分别取＋1叶测定壳寡糖诱导甘蔗叶多酚与防御酶（苯丙氨酸解氨酶、多酚氧化酶和过氧化物酶）活性的变化。【结果】50～75mg/L壳寡糖处理有利于甘蔗叶多酚积累;50mg/L壳寡糖处理2d后,苯丙氨酸解氨酶活性最大,为对照的1.8倍;50mg/L壳寡糖处理4d后,多酚氧化酶活性最高,为对照的1.7倍;75mg/L壳寡糖处理有利于提高甘蔗叶片过氧化物酶活性。【结论】50～75mg/L壳寡糖可以诱导甘蔗叶总多酚的积累及提高相关防御酶活性,这有利于提高甘蔗的整体抗性。
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| 关 键 词： | 甘蔗 壳寡糖 多酚 苯丙氨酸解氨酶 多酚氧化酶 过氧化物酶 |
Chitosan oligosaccharide induced changes in polyphenols and defense enzymes activities in sugarcane leaves |
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| ZHOU Gui,JIN Xiao-yun,DENG Guang-hui,QIU Li-hang ,ZOU Cheng-lin,LI Yang-rui,.Chitosan oligosaccharide induced changes in polyphenols and defense enzymes activities in sugarcane leaves[J].Guangxi Agricultural Sciences,2011(8):874-877. |
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| Authors: | ZHOU Gui JIN Xiao-yun DENG Guang-hui QIU Li-hang ZOU Cheng-lin LI Yang-rui |
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| Institution: | ZHOU Gui1,JIN Xiao-yun1,DENG Guang-hui1,QIU Li-hang 2,3,ZOU Cheng-lin1,LI Yang-rui2,4,5 * (1Guangxi University for Nationalities,Nanning 530007,China,2 State Key Laboratory for Subtropical Argo-bioresources Conservation and Utilization,3 Agricultural College,Guangxi University: Nanning 530005,5 Guangxi Crop Genetic Improvement and Biotechnology Lab,4 Sugarcane Research Center,Chinese Academy of Agricultural Sciences / Sugarcane Research Institute,Guangxi Academy of Agricultural Sc... |
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| Abstract: | Objective]The present study was carried out to explore the effect of chitosan oligosaccharide on secondary metabolites and some defense enzyme activities related to stress resistance in sugarcane. Method]The leaves of sugarcane variety ROC22 were treated with 0 (CK), 25, 50 and 75 mg/L chitosan oligosaccharide solution at the early elongation stage. The polyphenol content and the activities of defense enzymes, viz., phenylalanine ammonialyase (PAL), polyphenol oxidase (PPO) and peroxidase (POD) were measu... |
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| Keywords: | sugarcane chitosan oligosaccharide polypheols phenylalanine ammonialyase (PAL) polyphenol oxidase (PPO) peroxidase (POD) |
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