| 不同培养条件下两株溶磷真菌溶磷能力的比较 |
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| 引用本文： | 梁艳琼,雷照鸣,贺春萍,郑肖兰,余树华,郑服丛.不同培养条件下两株溶磷真菌溶磷能力的比较[J].广西农业科学,2011(8):825-830. |
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| 作者姓名： | 梁艳琼 雷照鸣 贺春萍 郑肖兰 余树华 郑服丛 |
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| 作者单位： | 海南大学环境与植物保护学院;中国热带农业科学院环境与植物保护研究所;中国热带农业科学院试验场;海南省热带作物病虫害生物防治工程技术研究中心/农业部热带农林有害生物入侵监测与控制重点开放实验室/海南省热带农业有害生物检测监控重点实验室; |
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| 基金项目： | 中央级公益性科研院所基本科研业务费专项项目(2009hzs1J015,2009hzs1J016); 国家科技支撑计划项目(2007BAD48B04-004); 公益性行业(农业)科研专项项目(nyhyzx07-033-2-3) |
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| 摘 要： | 【目的】探讨无机磷固、液体培养基及各种基本培养基、磷源等对两株溶磷真菌（FC和H3）溶磷能力的影响,以获得最适合这些菌株发挥溶磷潜力的培养条件。【方法】采用钼蓝比色法测定菌株在无机磷和有机磷液体培养基、简单培养基（SP）、NBRIY、NBRIP和NBRIYP培养基及含不同磷源（磷酸三钙、磷酸铁、磷酸铝、磷酸氢钙和有机磷）、不同浓度磷酸三钙（0.3%、0.5%、0.7%、0.9%和1.1%）培养基的溶磷能力。【结果】FC和H3菌株菌丝在PDA培养基上的最适培养时间为5d。分别在无机磷及有机磷液体培养基、简单培养基（SP）、NBRIY、NBRIP和NBRIYP培养基中培养7d后,FC菌株的溶磷量大小依次为NBRIY〉无机磷〉NBRIP〉SP〉有机磷〉NBRIYP,H3菌株的溶磷量大小依次为无机磷〉NBRIP〉SP〉NBRIY〉有机磷〉NBRIYP。以4种不同磷酸盐为磷源,FC菌株对磷酸盐的溶解能力依次为磷酸氢钙〉磷酸铝〉磷酸铁〉磷酸三钙,H3菌株溶解磷酸氢钙的能力较强,其次为磷酸三钙。在含不同浓度磷酸三钙的液体培养基中,两株菌株均能生长并具有溶磷能力,当磷酸三钙浓度为0.5%时,菌株溶磷能力最强。【结论】FC菌株的最适培养基为NBRIY,H3菌株的最适培养基为无机磷培养基。FC和H3菌株对难溶磷酸盐均有溶磷能力,而对磷酸氢钙的溶解能力较强;H3菌株对磷酸三钙的溶解能力较强。
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| 关 键 词： | 溶磷真菌 溶磷能力 培养基 磷源 |
Phosphate-solubilizing capacity of two fungal strains under different culture conditions |
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| LIANG Yan-qiong ,LEI Zhao-ming ,HE Chun-ping ,ZHENG Xiao-lan,YU Shu-hua,ZHENG Fu-cong ,.Phosphate-solubilizing capacity of two fungal strains under different culture conditions[J].Guangxi Agricultural Sciences,2011(8):825-830. |
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| Authors: | LIANG Yan-qiong LEI Zhao-ming HE Chun-ping ZHENG Xiao-lan YU Shu-hua ZHENG Fu-cong |
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| Institution: | LIANG Yan-qiong 1,2,LEI Zhao-ming 3,HE Chun-ping 1,4,ZHENG Xiao-lan1,YU Shu-hua3,ZHENG Fu-cong 1,2 * (1 College of Environment and Plant Protection,Hainan University,Danzhou,Hainan 571737,China,2 Environment and Plant Protection Institute,Chinese Academy of Tropical Agricultural Sciences,3 Testing Farm of Chinese Academy of Tropical Agricultural Sciences,4 Hainan Engineering Research Center for Biological Control of Tropica... |
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| Abstract: | Objective]The present experiment was aimed to study the effects of different inorganic solid and liquid media and phosphorus sources on phosphate-solubilizing capacity of two fungal strains to find out the best culture conditions for obtaining high potential of phosphate-solubilizing capacity. Method]The molybdenum blue colorimetry was used to determine the phosphate-solubilizing capacity of two fungal strains (FC and H3) in various culture media (inorganic or organic phosphorus liquid medium, SP, NBRIY, ... |
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| Keywords: | phosphate-solubilizing fungi phosphate-solublizing capacity culture medium phosphorus source |
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