广西野鸟H9N2亚型流感病毒的分离与鉴定

【目的】探讨H9N2亚型流感病毒在野鸟中的存在与传播,为广西流感疫情动态监测和防控及人流感预防监测提供重要依据。【方法】采集广西野鸟肺脏组织,将病料接种于9~11日龄SPF鸡胚尿囊腔进行分离培养,血清学试验鉴定HA和NA亚型,并克隆扩增其HA和NA基因。【结果】该分离株能被H9亚型阳性血清抑制,神经氨酸酶抑制（NI）试验结果将其鉴定为N2亚型,判断该病毒属于H9N2亚型流感病毒,命名为A/wildbird/Guangxi/H2/07,对HA和NA基因进行序列分析,发现与流感病毒H9亚型和N2亚型同源性最高,分别达到82.4%~99.0%和83.1%~99.9%。【结论】H9N2亚型流感病毒已在广西野鸟中存在,该结论为广西流感疫情动态监测和防控提供了重要依据。

Isolation and identification of H9N2 subtype influenza virus from wild bird in Guangxi

Objective]The present study was aimed to investigate the presence and spread of H9N2 subtype influenza virus in wild bird in order to understand its trends of occurrence and to develop proper control measures for preventing its infection on human beings in Guangxi. Method]The lung tissue was collected from wild birds of Guangxi, the virus was isolated and inoculated to 9-11 day chicken-Embryo. The HA and NA subtypes were identified using serological tests followed by amplification and sequencing of HA and NA genes . Result]The isolates were found to be inhibited by H9 subtypes positive serum, and identified as N2 subtype by neuraminidase test. This isolate belonged to the H9N2 subtype of avian influenza virus and named as A/wild bird/Guangxi/H2/07. The sequence analysis results of HA and NA genes showed 82.4-99.0% and 83.1-99.9% homologies of this virus strain with H9 and N2 subtypes, respectively. Conclusion]This study proved that the H9N2 subtype influenza virus exists in wild birds of Guangxi, and provided the reference for monitoring and control of Guangxi influenza epidemic situations.