澳洲荷斯坦奶牛MOET技术研究

为了快速扩繁良种澳洲荷斯坦奶牛的核心群,对澳洲荷斯坦奶牛MOET技术进行研究。结果表明,在不同月份超数排卵处理澳洲荷斯坦奶牛获得的总胚胎数和可用胚胎数,均无显著性差异（P〉0.05）;超数排卵处理不同生理阶段的澳洲荷斯坦奶牛,获得的总胚胎数差异不显著（P〉0.05）,但经产母牛获得的可用胚胎数明显多于青年母牛（P〈0.05）;而胚龄与受体母牛发情时间的同期性研究发现,桑椹胚和早期囊胚移植到发情后第7 d的受体母牛、囊胚移植到发情后第8 d的受体母牛、扩展囊胚移植到发情后第9 d的受体母牛,均可获得较高的胚胎移植受胎率和产犊率。

A study on multiple ovulation and embryo transfer (MOET) technology in Australian Holstein cow

The multiple ovulation and embryo transfer（MOET） technology was studied in Australian Holstein cows to widen the elite breeding of Australian Holstein core group.The results showed no significant differences in number of total embryos and available embryos of Australian Holstein cow as superovulated in different months.There was also a non significant difference in total embryos of superovulated Australian Holstein cow at different physiological stages,but available embryos of multiparity cow was found higher than that of young cow.Synchronism observation results of estrus and embryonic age showed that higher conception rate and calving rate of transplanted embryo were obtained when the morulae and early blastula were transplanted into the receptors in 7th day after estrus,the blastula were transplanted into the receptors in 8th day after estrus,and the expanded blastula were transplanted into the receptors in 9th day after estrus.