2011～2012年广西罗非鱼食源性致病菌及其药敏特性调查

目的]了解广西罗非鱼食源性致病菌的种类及其药敏特性,为研究制定罗非鱼食源性疾病的综合防控措施提供参考依据.方法]对2011～2012年采自广西罗非鱼主产区养殖场及市售罗非鱼样品（200份）进行沙门氏菌、志贺氏菌、致泻大肠埃希氏菌、金黄色葡萄球菌、单核细胞增生李斯特氏菌、副溶血性弧菌、无乳链球菌、海豚链球菌和致病性嗜水气单胞菌等9种重要食源性致病菌分离,采用Biolog自动微生物鉴定系统对分离获得的食源性致病菌进行鉴定,并参照美国临床实验室标准化研究所推荐的纸片琼脂扩散（K-B）法进行药敏试验.结果]所检测的9种食源性致病菌中除了未检出金黄色葡萄球菌、单核细胞增生李斯特氏菌和副溶血性弧菌外,其他6种食源性致病菌均有检出.养殖场罗非鱼样品的无乳链球菌和致病性嗜水气单胞菌检出率较高,分别为7.5％和5.0％,海豚链球菌、沙门氏菌、致泻大肠埃希氏菌和志贺氏菌的检出率分别为3.3％、2.5％、1.7％和0.8％;市售罗非鱼样品仅检出无乳链球菌、海豚链球菌、致病性嗜水气单胞菌和沙门氏菌,检出率分别为2.5％、1.3％、1.3％和1.3％.检出的食源性致病菌对常用抗菌药物表现出不同的敏感药物谱和耐药谱,以对头孢哌酮的敏感率最高,达56.7％;对青霉素、新霉素和林可霉素的耐药率最高,均为63.3％.结论]广西罗非鱼携带有以无乳链球菌和致病性嗜水气单胞菌为主的多种食源性致病菌,且检出的致病菌均存在多重耐药性.

An investigation on food borne pathogens and its antibiotic sensitivity isolated from tilapia in Guangxi during 2011-2012

Objective]The species of food borne pathogens and their antibiotic sensitivity in tilapia in Guangxi were investigated to provide useful information for monitoring and controlling food borne diseases.Method]Two hundreds tilapia samples were collected from tilapia farms and markets of the main tilapia production area in Guangxi during 2011-2012.The strains of Salmonella,ShigeHa,diarrheogenic Escherichia coli,Staphylococcus aureus,Listerra monocytogenes,Vibrio parahaemolyticus,Streptococcus agalactiae,Streptococcus iniae and pathogenic Aeromonas hydrophila among tilapia samples were isolated.The strains isolated from tilapia samples were identified by BIOLOG automatic microbiological assay system,and the antibiotic sensitivity tests of them were carried out according to the K-B method in performance standards for antimicrobial susceptibility testing of Clinical and Laboratory Standards Institute （CLSI）.Result]A total of 6 among 9monitored food borne pathogens were found.In samples from tilapia farms,Streptococcus agalactiae and pathogenic Aeromonas hydrophila had higher positive rate （7.5％ and 5.0％） than other pathogens.Streptococcus iniae,Salmonella,E.coli O157∶H7 and Shigella were 3.3％,2.5％,1.7％ and 0.8％,while in samples from markets,Streptococcus agalactiae,Streptococcus iniae,pathogenic Aeromonas hydrophila and Salmonella were found,and their positive rates were 2.5％,1.3％,1.3％ and 1.3％,respectively.The strains isolated from tilapia samples had different sensitivity and resist ance to 15 antimicrobial agents.Cefoperazone had the highest sensitive rate （56.7％）,while laevomycetin,fradiomycin and cillimycin had the highest resistant rate （63.3％）.Conclusion]There existed food borne pathogen contamination in tilapia in Guangxi mainly with Streptococcus agalactiae and pathogenic Aeromonas hydrophila,and all strains isolated from tilapia had multiple antibiotic resistance.