| 叶围微生物宏基因组文库的构建和分析 |
| |
| 引用本文： | 周亚奎,陈旭玉,郑服丛.叶围微生物宏基因组文库的构建和分析[J].广西农业科学,2008,39(3). |
| |
| 作者姓名： | 周亚奎 陈旭玉 郑服丛 |
| |
| 作者单位： | 1. 海南大学环境与植物保护学院,海南儋州,571737
2. 海南大学环境与植物保护学院,海南儋州,571737;中国热带农业科学院环境与植物保护研究所,海南儋州,571737 |
| |
| 基金项目： | 中央级公益性科研院所基本科研业务费专项资助项目 |
| |
| 摘 要： | 宏基因组文库技术是开发利用未培养微生物基因资源的有效途径。采用CTAB-SDS法直接从植物叶面沉积样品中提取基因组DNA,构建了以puc19为载体的基因组文库,共获得8126个阳性克隆,文库DNA总容量约30.1MB,平均插入片段长度为3.8kb。叶围微生物基因组文库的成功构建,对于以后研究植物和叶围微生物的关系有重要意义。
|
| 关 键 词： | 叶围微生物 宏基因组文库 构建 |
Construction and analysis of a metagenomic library on phyllosphere microbe |
| |
| ZHOU Ya-kui,CHEN Xu-yu,ZHENG Fu-cong.Construction and analysis of a metagenomic library on phyllosphere microbe[J].Guangxi Agricultural Sciences,2008,39(3). |
| |
| Authors: | ZHOU Ya-kui CHEN Xu-yu ZHENG Fu-cong |
| |
| Institution: | ZHOU Ya-kui1,CHEN Xu-yu1,ZHENG Fu-cong1,2 |
| |
| Abstract: | The metagenomic library technique is an effective method on the development and utilization of uncultured microorganism gene resources.By a combination of CTAB-SDS method,the genomic DNA of mixed were directly extracted from sediment samples of plant leaf surface.The metagenomic library using puc19 as vector was constructed and 8126 positive clones were obtained.The total number of metagenomic library DNA was about 30.1MB and the average insert size of DNA was about 3.8kb.The successful construction of metagenomic library of phyllosphere microbe would have great significance on studying the relationship between the plant and phyllosphere microbe in future. |
| |
| Keywords: | phyllosphere microbe metagenomic library |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
