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| 山羊痘病毒P32基因的克隆测序及序列分析 | |
| 引用本文： | 朱伟,冯淑萍,付薇,熊毅,刘棋.山羊痘病毒P32基因的克隆测序及序列分析[J].广西农业科学,2008,39(6). |
| 作者姓名： | 朱伟 冯淑萍 付薇 熊毅 刘棋 |
| 作者单位： | 1. 广西大学,南宁,530005;广西动物疫病预防控制中心,南宁,530001 2. 广西大学,南宁,530005 3. 广西动物疫病预防控制中心,南宁,530001 |
| 基金项目： | 广西科技攻关项目 |
| 摘 要： | 参考Genbank中的序列设计1对特异性引物对1株疫苗株和6株广西分离株的P32基因进行全基因序列测定和分析。结果显示，广西分离株间的核苷酸同源性为99．4％～100．0％，与疫苗株间的同源性为99．6％～99．9％，与Genbank中已发表山羊痘序列间的同源性为99．3％～99．8％；推导的氨基酸同源性为98．1％～100．0％、98．8％～99．7％、97．8％～99．4％；与国内疫苗毒株相比，广西分离株在100～105位缺失了6个碱基A，在651位所有广西分离株核苷酸的碱基全部由T变为C，647～652位核苷酸构成由TGTATA变异为TGTACA，多了1个限制性内切酶Bsp1407 I位点。研究结果为深入了解广西山羊痘分离株的分子特性以及山羊痘的诊断与防治奠定了基础。 |
| 关 键 词： | 山羊痘 P32基因 克隆 测序 |
Cloning and sequence analysis of P32 gene of goatpox virus |
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| ZHU Wei,FENG Shu-ping,FU Wei,XIONG Yi,LIU Qi.Cloning and sequence analysis of P32 gene of goatpox virus[J].Guangxi Agricultural Sciences,2008,39(6). | |
| Authors: | ZHU Wei FENG Shu-ping FU Wei XIONG Yi LIU Qi |
| Institution: | ZHU Wei1,2,FENG Shu-ping1,FU Wei2,XIONG Yi2,LIU Qi1,2(1 Guangxi University,Nanning 530005,China,2 Guangxi Center for Animal Disease Control , Prevention,Nanning,Guangxi 530001,China) |
| Abstract: | According to the sequence in GenBank,a pair of primers was designed and used for sequencing and analyzing the sequence of P32 gene of a vaccine strain and six isolated strains from Guangxi.The results showed that the homologies of nucleotide among the isolated strains,and between the isolated strains and the Chinese vaccine strain was 99.4%-100%,99.6%-99.9%,respectively;and that between the isolated strains and the strains published in GenBank was 99.3%-99.8%.Moreover,the homologies of deduced amino acid se... |
| Keywords: | goatpox P32 gene cloning sequencing |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |