草酸胁迫对甘蓝型油菜抗性相关蛋白含量和酶活性的影响

目的]研究草酸胁迫对甘蓝型油菜抗性相关蛋白含量和酶活性的影响,初步揭示草酸毒素对甘蓝型油菜致病性的生理生化机制,为油菜抗菌核病资源的初筛提供一种更方便、快速的方法.方法]以草酸溶液对苗期甘蓝型油菜材料GH01进行浸根处理,设置3个浓度（清水对照、1和2 mmol/L草酸溶液）、7个胁迫处理时间点（0、6、12、24、36、48和60h）,分别测定油菜叶片中可溶性物质含量和抗性相关蛋白酶酶活性.结果]草酸溶液胁迫下,随着胁迫时间增加,油菜叶片中可溶性蛋白和可溶性总糖含量不断增加,而抗性相关酶均呈先升后降趋势,酶活力曲线呈现“峰状”.其中,2 mmol/L草酸溶液胁迫下,SOD、PPO、POD和MDA含量分别在48、24、60和36 h达最大值,分别为359.66 U/g、257.50 U/g·min、44.94 U/g· min和72.86 μmol/L;在1 mmol/L草酸溶液胁迫下,SOD、PPO、POD和MDA含量分别在48、24、36和24 h达最大值,分别为302.17 U/g、215.00 U/g·min、24.01 U/g· min和75.75 μmol/L.结论]可利用草酸对甘蓝型油菜抗菌核病性进行初步鉴定,草酸溶液浓度以2 mmol/L为宜,其SOD、PPO、POD活性和MDA含量分别在处理48、24、60和36 h时达最高水平.

Content of protein related to resistance of cabbage type rape and enzymatic activity under oxalic acid stress

Objective]Effects of oxalic acid stress on content of protein related to resistance and enzymatic activity of cabbage type rape were studied to preliminarily reveal pathogenicity physiology and biochemistry mechanism of oxalic acid to cabbage type rape and offer a more convenient and faster method for screening anti-disease cabbage type rape resources. Method ]The root of oilseed rape （Brassiea napus L. ） was processed under the treatment of oxalic acid at different concen- trations （0, 1, 2 mmol/L） during the seedling stage of GH01. The content of soluble substances and the activities of several enzymes were measured in seven time intervals （0, 6, 12, 24, 36, 48 and 60 h）. Result]The results showed that both soluble protein and soluble carbohydrate increased all the time. However, the pathogenesis related protein enzyme went up first and then down with the enzyme activity curve presenting a peak form. Under the solution of 2.0 mmol/L oxalic acid, the maximal level of superoxide dismutase, polyphenol oxidase, peroxidase and malondialdehyde reached in 48,24,60 and 36 h, and their value was 359.66 U/g, 257.5 U/g-min, 44.94 U/g .rain and 72.86 μmol/L, respectively. Under the solution of 1.0 mmol/L oxalic acid, the maximal level of superoxide dismutase, polyphenol oxidase, peroxidase and malondialdehyde reached in 48,24,36 and 24 h, and their value was 302.17 U/g, 215 U/g·min, 24.01 U/g·min and 75.75 μmol/L, respec- tively. Conclusion ] Oxalic acid can be used for preliminary identification of the resistance of cabbage type rape to Sclerotinia and the suitable concentration of oxalic acid should be 2 mmol/L. The maximum value of superoxide dismutase, polyphenol oxidase, peroxidase and malondialdehyde reached the highest in 48, 24, 60 and 36 h.