叶蝉EST资源的微卫星信息分析

【目的】分析叶蝉EST序列信息，为其分子标记体系建立、遗传图谱构建及相关致害基因挖掘奠定基础。【方法】利用生物信息学技术对NCBI数据库中的4种叶蝉亚种EST序列进行SSR基序分析与统计，并对筛选出的SSR基序区段进行引物设计，开发出SSR标记。【结果】11044条叶蝉EST序列的总长为5866kb。按照引物筛选设定的参数，筛选获得1946条含有SSR基序的EST序列，占总EST序列的17．6％；其中包含2230个SSR基序并全部获得相应的SSR引物，平均间距为2．63kb。在2～5个核苷酸重复中，3个核苷酸重复为优势基序，占总基序的77．3％，尤以AAT类最丰富，占3个核苷酸重复总数的40．7％；双核苷酸重复中，AG类所占的比例最大，为39．1％。【结论】从叶蝉EST序列中筛选获得2230个SSR引物，以3个核苷酸重复基序所占比重最大。

Analysis of microsatellite information in EST resource of leafhopper

Objective]EST sequence information for leafhopper was analyzed to provide references for setting up molecular marker system, constructing genetic mapping and identifying associated injuring genes. Method ]Based on the four types of leafhopper subspecies EST sequences downloaded from NCBI data library, the SSR motifs and corresponding SSR primers were found through the bioinformatics method. Resuh]A total of 11044 EST sequences with the total length of 5866 kb was obtained. According to design parameters, 1946 EST sequences accounting for 17.6% of the total EST sequences were screened, which contained 2230 SSR motifs. Their corresponding SSR primers （average distance of 2.63 kb） were also obtained. In addition, trinucleotide was the major type of motifs in 2-5 nucleotide repeats, which accounted for 77.3%. Particularly, AAT motif was the most abundant among trinucleotide repeats and accounted for 40.7%. After that, AG motif was the largest among dinucleotide repeats and accounted for 39.1%. Conclusion]A total of 11044 leafhopper EST sequences which contained a large number of SSR motifs was analyzed. As a result, the trinucleotide motif type was the largest one and 2230 pairs of SSR primer were obtained.