六价铬（Cr-6＋）对福寿螺肝胰脏抗氧化酶活性和脂质过氧化的影响

目的]研究六价铬（Cr6＋）对福寿螺肝胰脏抗氧化酶活性和脂质过氧化的影响,为螺类的生理、生化研究及福寿螺的开发利用提供科学依据.方法]Cr6＋处理设0、1、5、10 mg/L浓度组,分别在处理后12、24、48、72和96 h取福寿螺肝胰腺进行超氧化物岐化酶（SOD）、过氧化氢酶（CAT）和丙二醛（MDA）含量测定.结果]在设计的剂量范围内,随着Cr6＋浓度的增加和暴露时间的延长,SOD的活性呈现先增高后降低的趋势,当处理时间为24 h、Cr6＋为10 mg/L时,肝胰脏中SOD活性达最大值,约为对照组2.1倍.在不同暴露浓度下,CAT活性总体上表现为低—高—低趋势,并随着暴露浓度和时间的延长,酶活力受到明显的抑制;1 mg/L浓度组CAT活性在暴露12 h后极显著增大为最大值,是对照的1.49倍,随后逐渐下降至对照水平附近并保持不变,但除了24和96 h外均无显著性变化;肝胰脏中MDA含量在整个试验期间均处于诱导状态,其中1、5 mg/L浓度组MDA含量分别于24、72 h无显著性变化,其余时间段均被显著或极显著诱导,而10 mg/L浓度组在整个试验期间均被显著诱导.结论]福寿螺肝胰脏中的SOD活性和MDA含量可以作为水生生态系统Cr污染的生物监测指标.

Effects of Cr6＋ on antioxidant enzyme activities and lipid peroxidation of hepatopancreatic in Pomacea canaliculata

Objective]An experiment was conducted to explore the effects of chrome （Cr6＋） exposure on antioxidant enzyme activities and lipid peroxidation of hepatopancreas to provide scientific references for physiological and biochemical research of snails as well as exploitation and utilization of Pomacea canaliculata. Method]The alien invasive species Po- macea canaliculata were exposed to Cr6＋ （1,5,10 rag/L） for 12, 24, 48, 72 and 96 h. The activities of superoxide dismutase （SOD） and catalase （CAT）, and the content of maglonydiadehyde （MDA） were measured by acute toxicity. Result]The results showed that within the designed dose range, the activities of SOD increased initially and decreased subsequently. In case of 10 mg/L Cr for 24 h, the SOD activity of hepatopancreas reached the highest, which was about 2.1 times that of the control group and the difference was extremely significant. In different exposure concentration, CAT activity in general showed the low - high - low trend, and with the increase of exposure concentration and time, the enzyme activity was ob- viously inhibited. The CAT activities in hepatopancreas, treated by lmg/L Cr for 12 h, attained to the maximum, which was 1.49 times that of the control group. Then it dropped gradually to the level of control group and maintained the same. Except for 24 and 96 h, there was no significant change. The concentration of MDA was induced during the whole expo- sure process. In 1 and 5 mg/L dose group, MDA content, in 72 and 24 h, respectively had no significant change. While at other time, it was significantly or extremely significantly induced. In 10 mg/L dose group, it was significantly induced during the whole test. Conclusion ] SOD activity and MDA content of Pomacea canaliculata in hepatopancreas can be used as aquatic ecosystem chrome pollution monitoring index.