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| 禽呼肠孤病毒分离株σ2蛋白基因克隆及序列分析 | |
| 引用本文： | 谢志勤,谢芝勋,邓显文,宋杨,温娟,刘加波,庞耀珊,谢丽基,蓝如束,张振荣,黄海强.禽呼肠孤病毒分离株σ2蛋白基因克隆及序列分析[J].广西农业科学,2010,41(9):977-980. |
| 作者姓名： | 谢志勤 谢芝勋 邓显文 宋杨 温娟 刘加波 庞耀珊 谢丽基 蓝如束 张振荣 黄海强 |
| 作者单位： | 1. 广西兽医研究所,南宁,530001 2. 南宁市动物卫生监督所,南宁,530001 3. 南宁市动物疾病预防诊断中心,南宁,530001 4. 广西疾病预防控制中心,南宁,530028 5. 武宣县动物疾病预防诊断中心,广西武宣,545900 |
| 基金项目： | 广西回国基金项目，广西自然基金项目 |
| 摘 要： | 为了解禽呼肠孤病毒（ARV）广西分离株与常用疫苗株在基因水平上的差异,将从广西某鸡场分离获得的2株ARV（R1、R2）分离株的σ2蛋白基因经RT-PCR扩增后,连接pMD18-T载体并转化大肠杆菌DH5α,重组质粒经PCR、双酶切鉴定及序列分析。结果表明,2株ARV分离株的σ2蛋白基因已成功克隆到质粒载体上;经序列分析发现,2株ARV分离株与ARV标准株S1133的核苷酸同源性分别高达99.2%和99.3%,其推导的氨基酸同源性分别高达98.1%和98.4%;两分离株间的核苷酸同源性为99.7%,其推导的氨基酸同源性为98.5%,具有很高的同源性。 |
| 关 键 词： | 禽呼肠孤病毒 分离株 σ2蛋白基因 序列分析 |
Cloning and sequence analysis of σ2 protein gene from avian reovirus isolates |
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| XIE Zhi-qin,XIE Zhi-xun,DENG Xian-wen,SONG Yang,WEN Juan,LIU Jia-bo,PANG Yao-shan,XIE Li-ji,LAN Ru-shu,ZHANG Zhen-rong,HUANG Hai-qiang.Cloning and sequence analysis of σ2 protein gene from avian reovirus isolates[J].Guangxi Agricultural Sciences,2010,41(9):977-980. | |
| Authors: | XIE Zhi-qin XIE Zhi-xun DENG Xian-wen SONG Yang WEN Juan LIU Jia-bo PANG Yao-shan XIE Li-ji LAN Ru-shu ZHANG Zhen-rong HUANG Hai-qiang |
| Institution: | 1 Guangxi Veterinary Research Institute,Nanning 530001,China;2 Nanning Animal Health Surveillance Institute,Nanning,Guangxi 530001,China;3 Nanning Prevention and Diagnostic Center of Animal Disease,Nanning,Guangxi 530001,China;4 Guangxi Center of Disease Control and Prevention,Nanning 530028,China;5 Wuxuan Center of Animal Disease Prevention and Diagnostic,Wuxuan,Guangxi 545900,China) |
| Abstract: | The experiment was conducted to study the difference between Guangxi and conventional strains of avian reovirus.Two avian reovirus（ARV） strains R1 and R2 were isolated from Guangxi and their σ2 protein gene were cloned into pMD18-T vector and transferred into DH5α after amplification by RT-PCR.After PCR,double-digestion and sequence analysis of the recombinant plasmid,it was observed that the σ2 protein gene had been cloned successfully into plasmid vector.The homologies of nucleic acids between Guangxi isolates R1,R2 and reference strain S1133 was 99.2 and 99.3%,respectively,and that of deduced amino acids was 98.1 and 98.4%,respectively.Moreover,the homology of nucleic acids and deduced amino acids between the strains R1 and R2 was found to be 99.7 and 98.5%,this proved that the two Guangxi strains showed high homology. |
| Keywords: | avian reovirus isolate σ2 protein gene sequence analysis |
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