| 云南拟单性木兰组织培养初步研究 |
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| 引用本文： | 甘露,李凯,王小青,陈雄庭.云南拟单性木兰组织培养初步研究[J].广西农业科学,2010,41(3):210-212. |
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| 作者姓名： | 甘露 李凯 王小青 陈雄庭 |
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| 作者单位： | 1. 中国热带农业科学院,海南海口,571101;海南大学,海口,570228
2. 中国热带农业科学院,海南海口,571101 |
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| 基金项目： | 国家科技基础条件平台建设资助项目(2005DKA21000-5-42) |
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| 摘 要： | 以不同季节的云南拟单性木兰幼嫩茎段为外植体，探讨外植体不同消毒时间、不同激素配比对腋芽萌发和生长以及芽增殖的影响。结果表明，取于6月份的木兰幼嫩茎段不适宜作为外植体进行培养；2．5％NaClO15min＋0．2％HgCl28min对外植体的消毒效果最好；最适的腋芽诱导培养基为MS＋6-BA0．5mg／L＋2，4-D0．1mg／L＋KT0．5mg／L＋蔗糖5％＋琼脂0．6％，增殖培养基为1／2MS＋6-BA0．5mg／L＋NAA0．1mg／L＋GA30．1mg／L＋蔗糖5％＋琼脂0．6％。
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| 关 键 词： | 云南拟单性木兰 腋芽 诱导 增殖 |
Tissue culture of Parakmeria yunnanensis Hu |
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| GAN Lu,LI Kai,WANG Xiao-qing,CHEN Xiong-ting.Tissue culture of Parakmeria yunnanensis Hu[J].Guangxi Agricultural Sciences,2010,41(3):210-212. |
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| Authors: | GAN Lu LI Kai WANG Xiao-qing CHEN Xiong-ting |
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| Institution: | 1 Chinese Academy of Tropical Agricultural Sciences;Haikou;Hainan 571101;China;2 Hainan University;Haikou 570228;China |
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| Abstract: | An experiment was undertaken to study the effects of different sterilization times(5,8,10 and 15 min) using 0.2% HgCl2 and hormone combinations on induction and growth and multiplication of axillary buds of Parakmeria yunnanensis Hu by using young shoots sampled in different months(April,June,and September) as explants.The results showed that young shoots sampled in June were unsuitable for tissue culture due to higher browning rate and lowest mortality rate.Sterilizing the shoots in 2.5% NaClO solution for... |
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| Keywords: | Parakmeria yunnanensis Hu axillary bud induction multiplication |
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