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| 牛分枝杆菌广西分离株ESAT-6基因鉴定及遗传进化分析 | |
| 引用本文： | 谢志勤,谢芝勋,刘加波,庞耀珊,邓显文,谢丽基,温娟,张振荣,黄海强.牛分枝杆菌广西分离株ESAT-6基因鉴定及遗传进化分析[J].广西农业科学,2010,41(8):827-830. |
| 作者姓名： | 谢志勤 谢芝勋 刘加波 庞耀珊 邓显文 谢丽基 温娟 张振荣 黄海强 |
| 作者单位： | 1. 广西兽医研究所,南宁,530001 2. 南宁市动物疾病预防诊断中心,南宁,530001 3. 武宣县动物疾病预防诊断中心,广西,武宣,545900 |
| 基金项目： | 新世纪百千万人才工程国家级人选专项基金项目，国家农业公益性专项项目，广西科技攻关项目，广西水产畜牧局科研计划项目 |
| 摘 要： | 根据GenBank中牛分枝杆菌（Mycobacterium bovis,M.bovis）的ESAT-6基因序列（No：BX248347）设计1对引物,PCR扩增获得2株牛分枝杆菌广西分离株（mt359、mt370）,纯化产物与pMD18-T载体连接后转化大肠杆菌DH5α,对经双酶切和PCR鉴定为阳性的重组质粒进行测序及同源性比较分析。结果表明,牛分枝杆菌广西分离株mt359、mt370与GenBank中已发表的6株毒株（5株结核分枝杆菌和1株牛分枝杆菌）的核苷酸同源性及推导的氨基酸同源性均为100.0%,与牛分枝杆菌标准株C680001的核苷酸同源性及推导的氨基酸同源性均为96.6%。说明致病性牛分枝杆菌的ESAT-6基因十分保守,不存在种间差异。 |
| 关 键 词： | 牛分枝杆菌 广西分离株 ESAT-6基因 PCR 同源性 |
Identification and phylogenetic analysis of ESAT-6 gene amongst Guangxi isolated strains of Mycobacterium boris |
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| XIE Zhi-qin,XIE Zhi-xun,LIU Jia-bo,PANG Yao-shan,DENG Xian-wen,XIE Li-ji,WEN Juan,ZHANG Zhen-rong,HUANG Hai-qiang.Identification and phylogenetic analysis of ESAT-6 gene amongst Guangxi isolated strains of Mycobacterium boris[J].Guangxi Agricultural Sciences,2010,41(8):827-830. | |
| Authors: | XIE Zhi-qin XIE Zhi-xun LIU Jia-bo PANG Yao-shan DENG Xian-wen XIE Li-ji WEN Juan ZHANG Zhen-rong HUANG Hai-qiang |
| Institution: | 1 Guangxi Veterinary Research Institute,Nanning 530001,China;2 Nanning Animal Disease Prevention and Diagnosis Center,Nanning,Guangxi 530001,China;3 Wuxuan Animal Disease Prevention and Diagnosis Center,Wuxuan,Guangxi 545900,China) |
| Abstract: | A pair of specific primers was designed using early secretary antigen target 6（ESAT-6） gene sequence（No： BX248347） of Mycobacterium bovine published in GenBank,and two Guangxi isolated strains of Mycobacterium bovine,viz.,mt359 and mt370 were identified using PCR amplification.The PCR products were purified and linked with pMD18-T vector,which was transferred to E.coli DH5α.The positive samples were sequenced after identified by enzyme digestion with Hind III and EcoR I and PCR.EAST-6 gene nucleotides and predicted amino acids were analyzed using DNAStar MegAlign software.The isolated strains mt359 and mt370 showed 100.0% homology with the 6 standard reference Mycobacterium strains from the GenBank,and 96.6% homology with standard Mycobacterium bovine strain C680001.These results indicated that pathogenic EAST-6 gene of Mycobacterium bovine is very conservative without any difference amongst species. |
| Keywords: | PCR |
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