| 不同性别类型苦瓜基因组DNA提取及RAPD标记初步研究 |
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| 引用本文： | 邓俭英,王绪,方锋学,张曼,孙德利.不同性别类型苦瓜基因组DNA提取及RAPD标记初步研究[J].广西农业科学,2007,38(3):223-226. |
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| 作者姓名： | 邓俭英 王绪 方锋学 张曼 孙德利 |
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| 作者单位： | 1. 广西农业科学院蔬菜研究中心,南宁,530007
2. 广西大学,南宁,530004 |
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| 基金项目： | 广西“新世纪十百千人才工程”专项(2003211)，广西青年科学基金项目(桂科青0447015)，广西农科院科技发展基金项目[2003006Z] |
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| 摘 要： | 采用改良的CTAB法成功地提取了不同性别类型苦瓜品种的基因组DNA,作为PCR扩增反应模板进行RAPD分析。结果表明,从200个10bp随机寡核苷酸引物中筛选到2个引物S30和S66,其可以在全雌性品种X-黑-d-d稳定地扩增出特异性条带,为下一步克隆苦瓜性别决定基因、探索苦瓜性别决定机理提供了试验依据。至于该特异性标记是否与苦瓜雌性性别有关,还有待进一步分析验证。
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| 关 键 词： | 苦瓜 性别类型 基因组DNA RAPD标记 |
| 文章编号： | 1002-8161（2007）03-0223-04 |
| 修稿时间： | 2006-12-05 |
Effective extraction of genomic DNA and RAPD analysis on different sex types of Momordica charantia L. |
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| DENG Jian-ying,WANG Xu,FANG Feng-xue,ZHANG Man,SUN De-li.Effective extraction of genomic DNA and RAPD analysis on different sex types of Momordica charantia L.[J].Guangxi Agricultural Sciences,2007,38(3):223-226. |
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| Authors: | DENG Jian-ying WANG Xu FANG Feng-xue ZHANG Man SUN De-li |
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| Institution: | 1. Vegetable Research Centre,Guangxi Academy of Agricultural Sciences, Nanning 530007, China 2. Guangxi University,Nanning 530004, China |
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| Abstract: | The genomic DNA was effectively extracted from different sex types of Momordica charantia L.by modified CTAB method,and employed as the template for PCR amplification reaction in RAPD analysis.A total of 200 arbitrary 10-meroligonucleotide primers were screened.Among them,two primers of S30 and S66 produced specific band in gynoecious cultivar X-Hei-d-d.The results provided the basis on cloning the sry gene of Momordica charantia L.andstudying the mechanism of sex-determining gene.Moreover,further studies on the relationship between specific band and female sex of Momordica charantia L.are expected. |
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| Keywords: | Momordica charantia L sex type genomic DNA RAPD analysis |
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