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基本培养基和激素组合对金钗石斛原球茎增殖的影响
引用本文:王国梅,韦鹏霄,岑秀芬.基本培养基和激素组合对金钗石斛原球茎增殖的影响[J].广西农业科学,2006,37(1):10-12.
作者姓名:王国梅  韦鹏霄  岑秀芬
作者单位:广西大学农学院,南宁,530005
基金项目:广西人事厅出国留学人员资助项目
摘    要:以野生金钗石斛茎段诱导长出的原球茎为材料,比较了M S、1/2 M S、B 5、M iller四种不同基本培养基在四个不同培养周期中金钗石斛原球茎生长增殖的情况,筛选出最佳增殖培养基为B 5培养基,原球茎的最适培养周期为30d;在不同生长素比较试验中,NAA对原球茎的增殖效果明显好于IBA;在不同激素6 BA和NAA配比组合正交试验中,采用Ducan多重比较法进行差异显著性分析表明,处理6 BA 0.5m g/L NAA 0.2m g/L的配比为最佳激素组合,原球茎净增殖倍数达到5.146倍。

关 键 词:金钗石斛  原球茎  基本培养基  生长素  激素组合
文章编号:1002-8161(2006)00-0010-03
收稿时间:2005-08-01
修稿时间:2005年8月1日

Effects of minimal media and plant hormone combinations on the protocorm multiplication of Dendrobium nobile
WANG Guo-mei,WEI Peng-xiao,CEN Xiu-fen.Effects of minimal media and plant hormone combinations on the protocorm multiplication of Dendrobium nobile[J].Guangxi Agricultural Sciences,2006,37(1):10-12.
Authors:WANG Guo-mei  WEI Peng-xiao  CEN Xiu-fen
Abstract:The growth and multiplication of protocorm induced from stem of wild Dendrobium nobile were compared when cultured in four different period and minimal media MS,1/2MS,B5 and Miller.The results showed that B5 medium was the best medium for the growth of protocorm of Dendrobium nobile,the best cultured period was 30d.The effect of NAA on multiplication of protocorm was superior to IBA in compared test of plant hormone.In the orthogonal test design of different 6-BA and NAA combinations,significance analysis of difference by Duncan's Multiple Range showed that 6-BA 0.5mg/L NAA 0.2 mg/L was the best for the growth of protocorm of Dendrobium nobile,and the multiplication times of protocorm was 5.146.
Keywords:Dendrobium nobile  protocorm  minimal media  auxin  hormone combinations
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