唇鱼骨肌间小骨的骨化过程

利用整体骨骼染色的方法,对唇(Hemibarbuslabeo)早期发育阶段肌间小骨的形态发生进行观察,结果表明:在受精后35 d(dpf)之前,除了肌间小骨,唇所有其它骨骼均已骨化完成。肌间小骨在35 dpf(相应的体长为23.67 cm)开始在尾部区域骨化,髓弓小骨首先出现在尾部的第37～41肌节之间,脉弓小骨首先出现在尾部的39～40肌节之间,然后依次往前;到62 dpf(相应的体长为30.03cm)肌间小骨骨化全部完成。骨化过程中,唇肌间小骨是从简单的I型,到卜型,再到Y型,再分化为各种复杂形态。唇肌间小骨出现的时机和形态形成规律与其它鲤科鱼类相似,提示鲤科各亚科鱼类肌间小骨的骨化过程可能受同样的遗传机制控制。研究亮点:目前关于鲤科鱼类肌间小骨骨化模式的研究仅限于鲢亚科的鲢和模式动物斑马鱼,通过比较其它亚科鱼类肌间小骨的骨化模式,有助于探讨鲤科鱼类肌间小骨骨化模式的保守性。本文发现亚科唇的肌间小骨骨化模式与鲢非常相似,提示鲤科肌间小骨的骨化过程可能受同样的遗传机制控制。

The ossificational process of the intermuscular bones in Hemibarbus labeo

To further understand the ossificational process of intermuscular bones in fishes from Cyprinidae,an important farming fish Hemibarbus labeo was investigated in this study.Intermuscular bones of H.labeo at different developmental stages were stained with alizarin red.Before 35 dpf(days post fertilization),all bones in H.labeo were ossified except for intermusclar bones.Since 35 dpf,intermuscular bones in tail area begun ossification in 23.67 cm standard length H.labeo.The ossified epineurals were observed in the myoseptums from 37thto 41th myotome,and the ossified epipleurals was only found between 39th to 40th myotome.As H.labeo was developping,ossified intermuscular bones were gradually observed in the myoseptums close to the head.Till to 62 dpf,all intermuscular bones were found ossified in H.labeo with 30.03 cm standard length.In addition,the primary morphology of intermuscular bone was type I,and ossified intermuscular bones with more complicated morphology were gradually forming from the type I during ossifing process.On the whole,the timing of intermucular bone initial ossification and the forming process of complex morphology in H.labeo were very similar to that in other species in Cyprinidae,indicating conserved genetic mechanism control of ossification of intermuscular bones might exist in Cyprinidae.