浅玫瑰色链霉菌壳聚糖酶罐发酵工艺及其优化

发酵罐装填系数0.6,控制罐压0.08 Mpa,采用单因素试验方法考察搅拌转速、种子液OD600、接种量和通气量4个因素对壳聚糖酶发酵的影响。根据试验结果确定该菌种10 L发酵罐发酵条件:搅拌转速250 r/min,种子液OD600=0.8~1.0,通气量2.0 vvm,接种量3%,其发酵周期56 h,酶活力在52 h达到最大,为(35.2±0.5)U/mL。补料工艺发酵周期为64 h,酶活力在60 h达到最大,为(46.4±0.5)U/mL。补料工艺能维持产酶期菌丝形态和延长产酶时间,从而提高产酶,最终补料工艺比批次发酵产酶提高了32%。该研究为壳聚糖酶工业化生产奠定了基础。

Optimization of chitosanase production from Streptomyces roseolus by fermentation process

Effects of agitator speed, aeration rate, inoculation amount and inoculum age on chitosanase production were studied by single factor experiments designs under the condition of 0.6 filling coefficient and 0.08Mpa tank pressure. The results showed that fermentation parameters in 10L tank were defined as following: 250r/min agitator speed, 3% inoculation amount, 2.0vvm aeration rate and seed solution of OD600=0.8-1.0. Under these conditions, the highest chitosanase activity in fermentation liquid was detected at 52h during 56h fermentation period, which reached 35.2±0.5U/mL. The maximum chitosanase activity was tested in 60h under a 64h fermentation period of feed batch fermentation, which reaches 46.4±0.5U/mL. The enzyme production of feed batch process increased 32% by maintain the mycelial morphology and extend the time of enzyme production stage. This article makes a contribution to industrial production of chitosanase.