长江口降海雌、雄鳗鲡血清生化指标和卵黄蛋白原的差异分析

比较了长江口降海洄游的鳗鲡雌、雄鱼血清中的14项生化指标及卵黄蛋白原含量的差异,并利用血清生化指标建立了相应的性别判定函数。结果发现,鳗鲡雌鱼血清中的甘油三酯、低密度脂蛋白、钙离子含量显著低于鳗鲡雄鱼(P0.05),白蛋白、葡萄糖含量显著高于鳗鲡雄鱼(P0.05),而谷丙转氨酶、谷草转氨酶、碱性磷酸酶、总胆红素、总蛋白、肌酐、总胆固醇、高密度脂蛋白胆固醇和磷酸根离子的含量在鳗鲡雌、雄鱼间无明显差异(P0.05)。此外,鳗鲡雌鱼的卵黄蛋白原含量显著高于鳗鲡雄鱼(P0.05)。根据不同生化指标,采用逐步选择法和全模型法分别建立了两个鳗鲡性别判定函数。

Differences of serum biochemical parameters and vitellogenin level between male and female Japanese eels Anguilla japonica collected at the Yangtze River Estuary

The differences of 14 serum biochemical indexes and vitellogenin content between female and male migratory Anguilla japonica collected at the Yangtze River Estuary were compared, and the function of sex identification on female and male eels were established. The results showed that serum triglyceride (TG), low density lipoprotein (LDL-C), and calcium (Ca²⁺) level of the male eels were significantly higher than those of the female eels (P<0.05), while the albumin (ALB), and glucose (Glu) of the female eels were significantly higher than those of the male eels (P<0.05).There were no significant differences between the female and male eels on the levels of Alanine transaminase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), total bilirubin (T-bil), total protein (TP), creatinine (CREA), total cholesterol (TC), high density lipoprotein cholesterol (HDL-C), and inorganic phosphorus (P) (P>0.05). Vitellogenin level of the female eels was significantly higher than that of the male eel (P<0.05). Base on the differences of the 14 serum biochemical indexes between female and male eels, two functions were established using stepwise discriminant analysis and entering independents together which could be used for sex determination of Anguilla japonica.