凡纳滨对虾Toll受体和溶菌酶mRNA组织表达的差异研究

采用荧光定量PCR技术,检测了凡纳滨对虾不同组织(血淋巴、鳃、肌肉和肝胰腺)中Toll受体和溶菌酶mRNA的相对表达量。结果表明:凡纳滨对虾Toll受体和溶菌酶mRNA在不同组织间的表达量存在显著差异。Toll受体mRNA在各组织中的相对表达水平由高到低顺序依次为血淋巴>鳃>肌肉>肝胰腺。溶菌酶mRNA表达水平则依次为血淋巴>鳃>肌肉>肝胰腺,但血淋巴和鳃之间无显著差异。溶菌酶mRNA和Toll mRNA表达量重复测定间的变异度在血淋巴和鳃组织间无显著差异。表明鳃和血淋巴是检测凡纳滨对虾Toll受体和溶菌酶mRNA的相对表达量的理想实验材料。研究亮点:Toll受体能够将异物入侵的信号从细胞外传递到细胞内并引起胞内信号级联反应产生各种免疫效益因子,因此其表达水平一定程度上可以反映对虾对病原入侵的灵敏性。溶菌酶活性是评判对虾免疫抗菌能力的重要指标。研究Toll受体和溶菌酶基因表达的组织差异性,可以为后续开展对虾营养免疫学研究中免疫相关基因检测时实验材料的选取提供理论基础。

Expressions of Toll receptor and lysozyme mRNA among tissues of white shrimp,Litopenaeus vannamei

The relative expression levels of Toll receptor and lysozyme mRNA in different tissues (haemolymph, gill, muscle and hepatopancreas) of the white shrimp, Litopenaeus vannamei, were quantitatively detected with real time PCR technology. The results indicated that the expressions of Toll receptor and lysozyme mRNA among tissues were significantly different. The relative expression of Toll receptor mRNA in haemolymph was significantly higher than that in gill as well as in muscle and hepatopancreas, and the relative expression of Toll receptor mRNA in gill was significantly higher than those in muscle and hepatopancreas. The relative expressions of lysozyme mRNA in haemolymph and in gill were significantly higher than those in muscle and hepatopancreas. While there was no significant difference between relative expressions of lysozyme mRNA in haemolypha and gill. There was no significant difference between the variation coefficients of Toll receptor and lysozyme mRNA expressions in haemolymph and in gill. It is therefore suggested that haemplymph and gill were the suitable material for assays of Toll receptor and lysozyme mRNA expression levels for L. vannamei.