| 免疫磁珠层析试纸法快速检测野生横纹东方鲀毒性 |
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| 引用本文： | 梁田田,卢玉平,潘迎捷,方国锋,林昕,黄天娇,卢瑛.免疫磁珠层析试纸法快速检测野生横纹东方鲀毒性[J].上海海洋大学学报,2018,27(6):924-929. |
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| 作者姓名： | 梁田田 卢玉平 潘迎捷 方国锋 林昕 黄天娇 卢瑛 |
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| 作者单位： | 上海海洋大学食品学院, 上海水产品加工贮藏工程技术研究中心, 上海 201306;农业部水产品贮藏保鲜质量安全风险评估实验室, 上海 201306,江苏中洋集团股份有限公司, 江苏 海安 226000,上海海洋大学食品学院, 上海水产品加工贮藏工程技术研究中心, 上海 201306;农业部水产品贮藏保鲜质量安全风险评估实验室, 上海 201306,上海海洋大学食品学院, 上海水产品加工贮藏工程技术研究中心, 上海 201306;农业部水产品贮藏保鲜质量安全风险评估实验室, 上海 201306,上海海洋大学食品学院, 上海水产品加工贮藏工程技术研究中心, 上海 201306;农业部水产品贮藏保鲜质量安全风险评估实验室, 上海 201306,上海海洋大学食品学院, 上海水产品加工贮藏工程技术研究中心, 上海 201306;农业部水产品贮藏保鲜质量安全风险评估实验室, 上海 201306,上海海洋大学食品学院, 上海水产品加工贮藏工程技术研究中心, 上海 201306;农业部水产品贮藏保鲜质量安全风险评估实验室, 上海 201306 |
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| 基金项目： | 上海市科学技术委员会部分地方院校能力建设项目（15320502100）；上海市科学技术委员会工程研究中心推广项目（16DZ2280300） |
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| 摘 要： | 调查并比较了野生横纹东方鲀(Takifugu oblongus)与养殖的菊黄东方鲀(Takifugu flavidus)、暗纹东方鲀(Takifugu obscurus)的毒性。随机选取17尾野生横纹东方鲀并用2%乙酸两次煮沸法提取各组织中河鲀毒素TTX,超滤离心去除杂蛋白,之后采用本实验室制备的免疫磁珠层析试纸条做定性检测,并对筛选出来的典型样本用国标小鼠生物法定量检测毒素含量与定性检测作对照,分析对比各组织TTX含量。野生横纹东方鲀肌肉和精巢TTX含量在0～7μg/g组织,由于个体差异处于无毒或弱毒水平;肝脏和卵巢TTX含量在4～400μg/g组织范围内,属于强毒水平;养殖菊黄东方鲀和暗纹东方鲀各组织均处于无毒水平。
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| 关 键 词： | 横纹东方鲀 毒性 TTX 小鼠生物法 免疫层析 |
| 收稿时间： | 2018/3/7 0:00:00 |
| 修稿时间： | 2018/4/30 0:00:00 |
Magnetic immunochromatographic method for rapid detection of toxicity inwild pufferfish (Takifugu oblongus) |
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| LIANG Tiantian,LU Yuping,PAN Yingjie,FANG Guofeng,LIN Xin,HUANG Tianjiao and LU Ying.Magnetic immunochromatographic method for rapid detection of toxicity inwild pufferfish (Takifugu oblongus)[J].Journal of Shanghai Ocean University,2018,27(6):924-929. |
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| Authors: | LIANG Tiantian LU Yuping PAN Yingjie FANG Guofeng LIN Xin HUANG Tianjiao and LU Ying |
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| Institution: | Shanghai Engineering Research Center of Aquatic-Product Processing & Preservation, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China;Laboratory of Quality & Safety Risk Assessment for Aquatic Products on Storage and Preservation, Ministry ofAgriculture, Shanghai 201306, China,Jiangsu Zhongyang Group Co., Ltd, Haian 226000, Jiangsu, China,Shanghai Engineering Research Center of Aquatic-Product Processing & Preservation, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China;Laboratory of Quality & Safety Risk Assessment for Aquatic Products on Storage and Preservation, Ministry ofAgriculture, Shanghai 201306, China,Shanghai Engineering Research Center of Aquatic-Product Processing & Preservation, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China;Laboratory of Quality & Safety Risk Assessment for Aquatic Products on Storage and Preservation, Ministry ofAgriculture, Shanghai 201306, China,Shanghai Engineering Research Center of Aquatic-Product Processing & Preservation, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China;Laboratory of Quality & Safety Risk Assessment for Aquatic Products on Storage and Preservation, Ministry ofAgriculture, Shanghai 201306, China,Shanghai Engineering Research Center of Aquatic-Product Processing & Preservation, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China;Laboratory of Quality & Safety Risk Assessment for Aquatic Products on Storage and Preservation, Ministry ofAgriculture, Shanghai 201306, China and Shanghai Engineering Research Center of Aquatic-Product Processing & Preservation, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China;Laboratory of Quality & Safety Risk Assessment for Aquatic Products on Storage and Preservation, Ministry ofAgriculture, Shanghai 201306, China |
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| Abstract: | |
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| Keywords: | Takifugu oblongus toxicity TTX mouse bioassay immunochromatography |
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