紫菜叶状体的红烂病研究

从福建省平潭岛野生坛紫菜群体中发现有患细菌性红烂病的叶状体,从患病的叶状体上分离得到病原细菌一株,经16S rDNA序列分析发现其与细菌域海螺菌目(Oceanospirillales)、盐单胞菌科(Halomonadaceae)、Cobetia属的海科贝特菌(Cobetia marina)的相似度达100%。病原细菌经液体培养基扩大培养后,将适量的菌液接种到不同种紫菜和坛紫菜不同品系的叶状体圆盘块上进行人工回复感染试验以确定其感染特性。结果发现该病原细菌能感染健康野生坛紫菜且出现的病症与海区患病坛紫菜相同,且该病原细菌对不同种紫菜(坛紫菜、条斑紫菜、未定名紫菜)的叶状体圆盘块和不同品系的坛紫菜(AN-2,YZ-6,JIU-7,ZS-1)叶状体圆盘块均能快速感染,所产生的病症与野生坛紫菜叶状体感染红烂病的症状相同:均出现了铁锈红色的死亡细胞,且同时含有少量被解离的单离细胞。上述结果说明分离到的病原细菌是坛紫菜红烂病的病原细菌,可以快速感染各种不同的紫菜叶状体。

Study on the red-rotting disease of Porphyra blades

A strain of pathogenic bacterium was isolated from the blades of wild type Porphyra haitanensis with red-rotting disease collected from the wild population along the coasts of Pingtan Island of Fujian Province.The bacterium had a 100% similarity with Cobetia marina(Oceanospirillales,Proteobacteria) according to analysis of the 16S rDNA array.After the pathogenic bacterium was cultured in liquid medium for a period of time,an amount of bacterial suspension was inoculated onto the blades of different Porphyra...