人工养殖对虾肠道内可培养细菌数量及组成分析

为深入了解人工养殖条件下养殖对虾肠道内菌群结构和携带病毒情况,应用常规细菌分离、培养与纯化,细菌16S rD NA序列分析的方法分析了我国山东、江苏、韩国不同养殖场的凡纳滨对虾(Litopenaeus vannamei)和中国明对虾(Fenneorpenaeus chinensis)肠道内可培养细菌的总数、优势菌组成和数量,并用巢式聚合酶链式反应(Nested polymerase chain reaction,Nested PCR)方法检测对虾携带病毒情况。结果显示各批次凡纳滨对虾和中国明对虾样品肠道内的可培养细菌总数在105~109cfu/g之间,并对分离出的优势菌进行属(种)鉴定,结果表明这些优势菌分别属于乳球菌属(Lactococcus sp.)、弧菌属(Vibrio sp.)、芽孢杆菌属(Bacillus sp.)、发光杆菌属(Photobacterium sp.)、希瓦氏菌属(Shewanella sp.)、节杆菌属(Arthrobacter sp.)、微小杆菌属(Microbacterium sp.)。凡纳滨对虾和中国明对虾均有样品检测为WSSV阳性,6批次WSSV阳性对虾样品中均检测到弧菌属细菌,占可培养细菌比例为33%~93.58%。2批次WSSV阳性对虾样品中检测到希瓦氏菌属细菌,占可培养细菌比例为21.67%~34.21%。4批次WSSV阳性对虾样品中检测到发光杆菌属细菌,占可培养细菌比例为21.03%~66.83%。

Analysis of the culturable bacteria's quantity and composition in the intestinal tract of cultivation shrimp

Abstract: In order to study the flora structure in the intestinal tract and carrying the virus condition of the different aquatic farm shrimp. we used conventional method of bacteria isolation, cultivation, purification and bacteria 16SrDNA sequences analysis to analyze the total number of culturable bacteria , the species and numbers of dominant bacteria and we used Nested polymerase chain reaction (Nested PCR) method to detect shrimp virus in Litopenaeus vannamei and Fenneorpenaeus chinensis cultivated in different farm of Jiangsu, Shandong and Republic of Korea. The results shown that the total number of culturable bacteria of Litopenaeus vannamei and Fenneorpenaeus chinensis is between 105 and 109 CFU/g, and identificated the dominant bacteria isolated from the intestinal tract to genus (species). The results shown that the dominant bacterial is respectively belong to Lactococcus sp, Vibrio sp, Bacillus sp, Photobacterium sp, Shewanella sp, Arthrobacter sp and Microbacterium sp. Litopenaeus vannamei and Fenneorpenaeus chinensis samples are tested positive for WSSV, 6 batches of shrimp samples which is tested positive WSSV, are isolated Vibrio sp from intestinal tract, occupied of culturable bacterial was 33%-93.58%, 2 batches of shrimp samples which is tested positive WSSV, are isolated Shewanella sp, from intestinal tract, occupied of culturable bacterial was 21.67%-34.21%, 4 batches of shrimp samples which is tested positive WSSV, are isolated Photobacterium sp, from intestinal tract, occupied of culturable bacterial was 21.03%-66.83%.