DNA条形码在鱼类胃含物种类鉴定中的应用

DNA条形码技术是利用一段较短的DNA序列实现快速准确物种鉴定的工具,已成为近年来生物类群的研究热点,并逐步被引进到各个领域。在海洋生物群落生态学中,DNA条形码已被用于海洋生物系统分类、分子遗传多样性、种间亲缘关系、系统进化关系等研究;国外已有学者将其应用到海洋生物食性分析的研究中,国内相关文章较少。本文介绍了DNA条形码的由来、发展以及相关原理和基本实验步骤,并阐述了其优点以及局限性,分析了其在肉食性鱼类胃含物不可辨认种类鉴定中的应用价值,并展望其在未来分类学发展中的应用前景。

Application of DNA barcoding in species analysis of fish stomach content

DNA barcoding (a technology to provide rapid, accurate and automated species identifications by using short orthologous DNA sequences) has been fundamentally important in the past few years in system classification, molecular genetic diversity and species relationships of marine organisms as well as evolutionary relationships, and so on. The data set in DNA barcoding is obtained from a single specimen without regard to life stage or morphological characters of targets. Many a times foreign scholars elucidate the impact of some predation on prey populations using DNA barcoding nevertheless we did less. Herein we introduce DNA barcodes, then discuss the relative merits in relation to the utility of DNA barcoding in identifying prey items of fish diets. The DNA barcoding shows promise in providing a practical, species-level identification tool that can be used for taxonomy and ecological studies, and many more.