乙酰甲喹对刺参幼参非特异性免疫、生长及抗应激能力的影响

选用初始体重为0.6 g左右的幼参,水体中分别添加0(对照组)、0.5、1.0、2.0、4.0和6.0 mg/L的乙酰甲喹。前69天每两天全部换水后,按照用药浓度泼洒药物一次。停药两周后经长途运输,考察其抗应激能力。实验结果表明:前30 d,各组刺参体壁中SOD酶活性都呈现增高趋势,2.0 mg/L组SOD活性达到最高,之后又呈现降低趋势;随着乙酰甲喹浓度的增加,各实验组刺参体壁中AKP和ACP酶活性呈现先增加后降低的趋势,2.0 mg/L和4.0mg/L组刺参酶活性最高;40 d后各实验组刺参体壁酶活性逐渐与对照组持平。所有实验组刺参的末体重、增重率和特定生长率均显著高于对照组(P0.05),4.0 mg/L组刺参末体重、增重率和特定生长率最高,显著高于其他各组(P0.05);4.0 mg/L组成活率为90.6%,显著高于其他各组(P0.05)。高浓度(6.0 mg/L)的乙酰甲喹导致幼参抗应激能力差。综合以上分析,认为刺参保苗期间,乙酰甲喹的适宜添加量为2.0~4.0 mg/L。

Effects of mequindox on non-specific immunity, growth performance and stress resistance of juvenile sea cucumber Apostichopus japonicus

The present study aimed to evaluate the effects of mequindox on non-specific immunity, survival rate, growth performance and stress resistance in juvenile sea cucumber (Apostichopus japonicus) breeding. The sea cucumber with initial body weight of 0.6g were chosen, and fed in different mequindox solutions of 0 (control group), 0.5, 1, 2, 4 and 6 mg/L for 69 days. The resistance after long-distance transportation was investigated two weeks after the drug withdrawal. The results showed that the AKP, ACP and SOD enzyme activities of juvenile sea cucumber increased significantly after the use of mequindox, and reached peak in the 2, 4 mg/L mequindox solutions and then decreased as the mequindox continued to increase, initially. However, after 40 days, the enzyme activities in the experimental groups generally decreased to the values in the control group. Appropriate amouts of mequindox can increase the growth rate and survival rate of the sea cucumber. The final body weight (FBW), weight gain rate (WGR) and specific growth rate (SGR) in the experimental groups were significantly higher than those in the control group (P<0.05). The FBW, WGR and SGR were the biggest in the 4 mg/L mequindox solution with the biggest survival rates (90.6%), significantly higher than the other groups (P<0.05). Besides, high concentration of mequindox (6 mg/L) decreased the stress resistance of juvenile sea cucumber. Our study suggested that the proper amount of mequindox for juvenile sea cucumber in the period of growth seeding was approximately 2~4 mg/L in relatively short period (< 40 days).